Cathodes enhance Corynebacterium glutamicum growth with nitrate and promote acetate and formate production.
Cathodes enhance Corynebacterium glutamicum growth with nitrate and promote acetate and formate production.
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DOI：
10.1016/j.biortech.2016.05.051
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发表时间：
2016-09
影响因子：
11.4
通讯作者：
Nikolaos Xafenias;C. Kmezik;Valeria Mapelli
中科院分区：
文献类型：
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作者：
Nikolaos Xafenias;C. Kmezik;Valeria Mapelli
工业上重要的谷氨酸棒杆菌只能不完全地将硝酸盐还原成亚硝酸盐，然后亚硝酸盐积累并抑制生长。在此，我们报告阴极可以解决这个问题，并通过促进亚硝酸盐还原来增强葡萄糖发酵和生长。细胞生长在相对高的电位下受到抑制，但当电位更还原时（-1.20 V，蒽醌-2-磺酸盐作为氧化还原介体或-1.25 V vs. Ag/AgCl），细胞生长受到显著抑制。在这些条件下，葡萄糖的消耗快6倍，乙酸酯的产量高11倍（高达1.1 mol/mol葡萄糖）。醋酸盐浓度是迄今为止C.在厌氧条件下，谷氨酸的含量可达5.3 ± 0.3g/L。本文还首次证明了C.谷氨酸在强还原条件下，我们把这归因于一个可能的机制CO2生物还原，电化学触发。
The industrially importantCorynebacterium glutamicumcan only incompletely reduce nitrate into nitrite which then accumulates and inhibits growth. Herein we report that cathodes can resolve this problem and enhance glucose fermentation and growth by promoting nitrite reduction. Cell growth was inhibited at relatively high potentials but was significant when potentials were more reductive (−1.20 V with anthraquinone-2-sulfonate as redox mediator or −1.25 V vs. Ag/AgCl). Under these conditions, glucose was consumed up to 6 times faster and acetate was produced at up to 11 times higher yields (up to 1.1 mol/mol-glucose). Acetate concentrations are the highest reported so far forC. glutamicumunder anaerobic conditions, reaching values up to 5.3 ± 0.3 g/L. Herein we also demonstrate for the first time formate production (up to 3.4 ± 0.3 g/L) byC. glutamicumunder strongly reducing conditions, and we attribute this to a possible mechanism of CO2bioreduction that was electrochemically triggered.