Expression of multidrug resistance protein gene in patients with glioma after chemotherapy
Expression of multidrug resistance protein gene in patients with glioma after chemotherapy
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DOI：
10.1023/a:1005954406809
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发表时间：
1998-10-01
影响因子：
3.9
通讯作者：
Hori, S
中科院分区：
文献类型：
--
作者：
Abe, T;Mori, T;Hori, S
两种不同的 ATP 结合膜糖蛋白，170 kDa P-糖蛋白 (P-gp) 和 190 kDa 多药耐药蛋白 (MRP)，参与癌细胞多药耐药表型的获得。当用抗癌药物治疗时，在各种人类肿瘤中经常观察到 P-gp 的过度表达。在这项研究中，我们询问癌症化疗后人类神经胶质瘤中 MRP 是否过度表达。我们研究了胶质瘤患者肿瘤样本化疗前后 MRP 和 P-gp 的表达。 23 名未治疗患者中，有 16 名（70%）观察到 MRP 表达，16 名 MRP 阳性患者中 MRP 阳性细胞占整个细胞群的比例为 3% 至 32%。 23名患者中有4名（18％）观察到P-gp阳性肿瘤，整个细胞群中P-gp阳性细胞的比例范围为4％至23％。与化疗前相比，所有接受检查的患者化疗后MRP阳性或P-gp阳性胶质瘤细胞的比例均有所增加。我们观察到 MRP 或 P-gp 的表达与肿瘤分级之间没有统计学上显着的相关性。这些结果表明，MRP 以及 P-gp 可能与人类神经胶质瘤的获得性或内在耐药性有关。
Two different ATP-binding membrane glycoproteins, the 170 kDa P-glycoprotein (P-gp) and the 190 kDa multidrug resistance protein (MRP), are involved in the acquisition of multidrug resistance phenotypes in cancer cells. Overexpression of P-gp is often observed in various human tumors when treated with anticancer agents. In this study, we asked whether MRP was overexpressed in human gliomas after cancer chemotherapy. We investigated expression of MRP and P-gp before and after chemotherapy in tumor samples from patients with glioma. MRP expression was observed in 16 (70%) of 23 untreated patients, and the proportion of MRP-positive cells in the whole cell population ranged from 3 to 32% in the 16 MRP-positive patients. P-gp-positive tumors were observed in 4 (18%) of 23 patients, and the proportional rates of P-gp-positive cells in the whole cell population ranged from 4 to 23%. The proportional rate of MRP-positive or P-gp-positive glioma cells increased after chemotherapy when compared with that before chemotherapy in all patients examined. We could observe no statistically significant correlation between expression of MRP or P-gp and tumor grade. These results suggest that MRP as well as P-gp may be involved in acquired or intrinsic drug resistance in human glioma.