Differential expression of the H-ras mutated and normal alleles in rabbit DMBA-induced keratoacanthomas.
Differential expression of the H-ras mutated and normal alleles in rabbit DMBA-induced keratoacanthomas.
复制标题
兔 DMBA 诱导的角化棘皮瘤中 H-ras 突变和正常等位基因的差异表达。
DOI：
10.1002/ijc.2910610515
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发表时间：
1995
影响因子：
6.4
通讯作者：
Pellicer,A
中科院分区：
文献类型：
--
作者：
Matesanz,F;Oliva,MR;Villamarin,A;Kamino,H;Pellicer,A
角化棘皮瘤(KAS)是一种良性的、自我退化的肿瘤，在人类和实验模型中都观察到了H-rasoncogene突变的高发生率。为了确定在7，12-二甲基苯并(A)菲(DMBA)诱导的兔皮肤角化瘤(KAS)中突变的H-ras等位基因相对于其正常对应基因的表达水平，我们利用了一种基于逆转录聚合酶链式反应(RT-PCR)和H-rasgene突变分子选择性切割的定量技术。对16个Kas的分析表明，在不同的肿瘤中，突变的H-ras转录本的丰度是未突变的H-ras转录本的3倍。突变等位基因的这种高表达似乎与KA分化增加相关，进而可能有助于肿瘤的消退。©1995 Wiley-Liss公司
Keratoacanthomas (KAs) are benign and self‐regressing tumors in which a high incidence of the mutated H‐rasoncogene has been observed both in humans and in experimental models. To determine the level of expression of the mutated H‐rasallele with respect to its normal counterpart in 7,12‐dimethylbenz(a)anthracene (DMBA)‐induced KAs in rabbit skin, we have utilized a quantitative technique based on reverse transcription polymerase chain reaction (RT‐PCR) and selective cleavage of the mutated molecules of the H‐rasgene. Analysis of 16 KAs showed that the mutated H‐ras transcripts were up to 3‐fold more abundant than the non‐mutated H‐ras transcript in the different tumors. This higher expression of the mutated allele appears to correlate with increased differentiation in the KAs and in turn may contribute to tumor regression. ©1995 Wiley‐Liss, Inc.