Atypical Toxoplasma gondii genotypes identified in oocysts shed by cats in Germany
Atypical Toxoplasma gondii genotypes identified in oocysts shed by cats in Germany
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DOI：
10.1016/j.ijpara.2009.08.001
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发表时间：
2010-03-01
影响因子：
4
通讯作者：
Schares, G.
中科院分区：
文献类型：
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作者：
Herrmann, D. C.;Pantchev, N.;Schares, G.
在2007年6月至2008年12月期间，德国共收集了18259份猫粪便样本，并分析了是否存在刚地弓形虫卵囊。1 - 6月间采集的弓形虫阳性样本比例明显低于7 - 12月。刚地弓形虫卵囊脱落猫的年龄与阴性对照猫的年龄无显著差异。采用newSAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico等9个限制性片段长度多态性（RFLP）标记对46份弓形虫阳性样本进行了遗传特征分析。此外，利用PCR-RFLP标记c22-8、c29-2、L358、PK1和Apico进一步分型了22株已在先前研究中部分鉴定的分离株。对68株弓形虫分离株进行种族分型分析发现，大多数（54株）弓形虫分离株在所有位点均为II型模式，但在Apico位点显示I型模式。3个分离株在包括Apico位点在内的所有位点均表现为II型。此外，我们在所有位点检测到1株克隆型III型和3株非典型和混合基因型。7株分离株不能完全基因分型。其中一个分离株在Apico位点显示I型和II型等位基因。据我们所知，这是首次描述在自然感染的猫的粪便中存在与克隆型I、II和III不同的弓形虫基因型。(C) 2009年澳大利亚寄生虫学学会Elsevier Ltd.出版。版权所有。
A total of 18,259 feline faecal samples from cats in Germany were collected and analysed for the presence of Toxoplasma gondii oocysts between June 2007 and December 2008. The proportion of T. gondii-positive samples collected between January and June was significantly lower than between July and December. The age of cats shedding T. gondii oocysts was not significantly different from the age of negative control cats. Forty-six T. gondii-positive samples were genetically characterised using nine PCR-restriction fragment length polymorphism (RFLP) markers which included newSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. In addition, 22 isolates that had already been partially characterised in a previous study were further typed using PCR-RFLP markers c22-8, c29-2, L358, PK1 and Apico. Genocyping of the 68 isolates revealed that the majority of T. gondii isolates (n = 54) had Type II patterns at all loci but displayed a Type I pattern at the Apico locus. Three isolates displayed Type II patterns at all loci, including the Apico locus. In addition, we detected one isolate with clonal Type III patterns at all loci and three isolates with atypical and mixed genotypes. Seven isolates could not be fully genotyped. One of those isolates displayed alleles of both Types I and II at the Apico locus. To our knowledge this is the first description of the presence of T. gondii genotypes different from the clonal Types I, II and III in the faeces of naturally infected cats. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.