Antigen/major histocompatibility complex-specific activation of murine T cells transfected with functionally rearranged T-cell receptor genes.
Antigen/major histocompatibility complex-specific activation of murine T cells transfected with functionally rearranged T-cell receptor genes.
复制标题
抗原/主要组织相容性复合物特异性激活转染功能重排 T 细胞受体基因的鼠 T 细胞。
DOI：
10.1073/pnas.84.21.7614
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发表时间：
1987
影响因子：
11.1
通讯作者：
Hood,L
中科院分区：
文献类型：
--
作者：
Kuo,CL;Hood,L
从细胞色素c特异性的主要组织相容性复合体(MHC)限制性的小鼠T细胞杂交瘤中分离出编码T细胞抗原受体α链和β链的基因，通过电穿孔将其导入辅助系小鼠T细胞系。为了检测这些基因产物对抗原和/或MHC特异性的贡献，对所得到的转染物进行了功能抗原和/或MHC识别测试。只有那些同时表达由正常T细胞贡献的引入基因(α和β)的转染者才能对适当的抗原/MHC对产生特异性反应。只表达正常T细胞的一个引入基因(α或β)或配对的杂交基因(即一个来自正常T细胞，另一个来自融合伙伴)的转染者，都不能对供体T细胞识别的相同抗原和MHC产物的组合产生反应。然而，一个表达了编码融合伙伴的α和β链的转基因基因的克隆即使在没有抗原的情况下也显示出对抗原提呈细胞的反应性。这些数据表明，T细胞受体的α-β异源二聚体是定义T细胞的精细特异性所必需的。
The genes encoding the alpha and beta chains of the T-cell antigen receptor isolated from a cytochrome c-specific, major histocompatibility complex (MHC)-restricted murine T-cell hybridoma were introduced into a mouse T-cell line of helper lineage by electroporation. In order to examine the contributions of those gene products to antigen and/or MHC specificity, the resultant transfectants were tested for functional antigen and/or MHC recognition. Only those transfectants that express both the introduced genes (alpha and beta) contributed by the normal T cell can respond specifically to the appropriate antigen/MHC pair. None of the transfectants that express only one of the introduced genes (alpha or beta) of the normal T cell, or paired hybrid genes (i.e., one gene from the normal T cell and the other from the fusion partner), can respond to the same combination of antigen and MHC product recognized by the donor T cell. However, one clone expressing the transfected genes that encode the alpha and beta chains of the fusion partner shows reactivity to the antigen-presenting cells even in the absence of the antigens. These data suggest that the alpha beta heterodimer of the T-cell receptor is required to define the fine specificity of a T cell.