OXYGEN AVAILABILITY AND GROWTH OF THE CHICK-EMBRYO

OXYGEN AVAILABILITY AND GROWTH OF THE CHICK-EMBRYO
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DOI:
10.1016/0034-5687(81)90091-8
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发表时间:
1981-01-01
期刊:
RESPIRATION PHYSIOLOGY
影响因子:
--
通讯作者:
WELCH, JE
WELCH, JE
中科院分区:
其他
文献类型:
--
作者:
METCALFE, J;MCCUTCHEON, IE;WELCH, JE

文献摘要

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使用在 50% 湿度、38 摄氏度下孵化的白来航鸡蛋进行了两个实验。 C 和平均气压 747 托。在一项实验中,实验组中的鸡蛋各半覆盖着氯丁橡胶膜,从而减少了扩散能力。 20%,并在 21% O2 中孵育。在第二个实验中,实验蛋在 60% O2 中孵化(不加盖)。在每项研究中,对照蛋与实验蛋同时在 21% O2 中孵化(无遮盖)。所有 3 组的蛋表面积(根据新产蛋的重量计算)和 18 天时胚胎重量之间的关系均得到证实。当进行各组之间的比较时,发现半覆盖的鸡蛋的胚胎生长显着延迟;在 60% O2 中孵化的鸡蛋胚胎在 18 天时明显比在空气中孵化的对照鸡蛋重。通过在 60% O2 中孵化卵子可以加速胚胎生长,这一事实表明,离开绒毛尿囊毛细血管的血液中的 O2 张力通常会限制该物种孵化前 18 天的胚胎生长速度。
Two experiments were performed using White Leghorn eggs incubated in 50% humidity, at 38.degree. C and an average barometric pressure of 747 torr. In 1 experiment eggs in the experimental group were each half covered with a neoprene membrane, reducing the diffusing capacity by .apprx. 20%, and incubated in 21% O2. In the 2nd experiment the experimental eggs were incubated (uncovered) in 60% O2. Control eggs were incubated (uncovered) in 21% O2 simultaneously with the experimental eggs in each study. A relationship between egg surface area (calculated from weight of the freshly laid egg) and embryo weight at 18 days was confirmed for all 3 groups. When comparisons were made among the groups, there was significant retardation of embryonic growth in the half-covered eggs; embryos from eggs incubated in 60% O2 were significantly heavier at 18 days than control eggs incubated in air. The fact that embryo growth can be accelerated by incubation of the egg in 60% O2 suggests that the O2 tension in blood leaving the chorioallantoic capillaries normally limits the rate of embryonic growth for the first 18 days of incubation in this species.