THE NUCLEOTIDE-SEQUENCE OF THE DNA-LIGASE GENE (CDC9) FROM SACCHAROMYCES-CEREVISIAE - A GENE WHICH IS CELL-CYCLE REGULATED AND INDUCED IN RESPONSE TO DNA DAMAGE
THE NUCLEOTIDE-SEQUENCE OF THE DNA-LIGASE GENE (CDC9) FROM SACCHAROMYCES-CEREVISIAE - A GENE WHICH IS CELL-CYCLE REGULATED AND INDUCED IN RESPONSE TO DNA DAMAGE
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DOI：
10.1093/nar/13.23.8323
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发表时间：
1985-12-09
影响因子：
14.9
通讯作者：
JOHNSTON, LH
中科院分区：
文献类型：
--
作者：
BARKER, DG;WHITE, JHM;JOHNSTON, LH
本文报道了一个含有聚合转录的CDC 9和CDC 36基因的3.85kb的DNA片段的核苷酸序列。S1核酸酶图谱显示CDC 9 mRNA的主要5′端，以及3′多聚腺苷酸化的一个主要位点和一个次要位点。这两个位点位于CDC 36基因的C-末端编码区，这意味着这两个基因是从重叠序列转录的。CDC 9基因的一个有趣的结构特征是一系列6个六核苷酸重复序列（ATGATT），其发生在转录起始位点上游650 bp内。这些重复元件可能与细胞分裂周期调控的CDC 9表达有关。酵母DNA连接酶（Mr 84，806）的预测氨基酸序列与T4和T7噬菌体DNA连接酶的序列的比较显示，除了包含3个短同源片段的约45个氨基酸的延伸之外，几乎没有相似性。该区域可能代表多核苷酸连接酶共有的ATP结合结构域。
TheCDC9gene ofSaccharomycescerevisiaeencodes a DNA ligase, and we have determined the nucleotide sequence of a 3.85 kb fragment of DNA which emcompasses the convergently transcribedCDC9andCDC36genes. S1 nuclease mapping has revealed a major 5′ end for the CDC9 mRNA, and one major and one minor site for 3′ polyadenylation. These two sites lie within the C-terminal coding region of theCDC36gene, implying that these two genes are transcribed from overlapping sequences. An interesting structural feature of theCDC9gene is a series of 6 hexanucleotide repeats (ATGATT) which occur within the 650 bp immediately upstream from the site of transcription initiation. These repeat elements may be implicated in the cell division cycle regulated expression ofCDC9. Comparison of the predicted amino acid sequence of the yeast DNA ligase (Mr 84,806) with the sequences of the T4 and T7 bacteriophage DNA ligases reveals little similarity except for a stretch of approximately 45 amino acids, comprising 3 short homologous segments. This region may represent an ATP-binding domain common to polynucleotide ligases.