ROLE OF BASIC FIBROBLAST GROWTH-FACTOR IN VASCULAR LESION FORMATION
ROLE OF BASIC FIBROBLAST GROWTH-FACTOR IN VASCULAR LESION FORMATION
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DOI：
10.1161/01.res.68.1.106
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发表时间：
1991-01-01
影响因子：
20.1
通讯作者：
REIDY, MA
中科院分区：
文献类型：
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作者：
LINDNER, V;LAPPI, DA;REIDY, MA
在本研究中，我们研究了碱性成纤维细胞生长因子（bFGF）是否在平滑肌细胞（SMCs）对剥脱损伤的增殖反应中起作用。 大鼠颈动脉平滑肌中发现表达bFGF的mRNA，bFGF蛋白被发现存在于大鼠主动脉中的免疫印迹分析。 系统地给予bFGF是一种有效的有丝分裂原血管平滑肌细胞在动脉剥脱与球囊导管，增加复制从11.5%的控制到54.8%。 用器械（细丝环）剥脱，仅对内侧SMC造成最小损伤，在bFGF输注后显示出类似的复制增加（1.3%对43.3%）。 然而，在未操作的血管中，SMC对注入的bFGF无反应。 输注“丝裂毒素”（bFGF结合皂草素）导致球囊损伤后动脉壁中存活SMC数量减少50%以上。 球囊损伤后，bFGF的长期给药（12 μ g/天，持续2周）导致内膜增厚增加约2倍。 这些结果表明，碱性成纤维细胞生长因子，这是合成的动脉壁，可能是一个有效的有丝分裂原SMC在体内，并建议，任何释放内源性碱性成纤维细胞生长因子可能能够刺激SMC增殖，这可能随后导致内膜病变的形成。
In the present study we investigated whether basic fibroblast growth factor (bFGF) plays a role in the proliferative response of smooth muscle cells (SMCs) to denuding injury. Rat carotid smooth muscle was found to express the mRNA for bFGF, and bFGF protein was found to be present in rat aorta by immunoblot analysis. Systematically administered bFGF was a potent mitogen for vascular SMCs in arteries denuded with a balloon catheter, increasing replication from 11.5% in controls to 54.8%. Denudation with a device (filament loop), which causes only minimal damage to medial SMCs, showed a similar increase in replication (1.3% versus 43.3%) after bFGF infusion. In unmanipulated vessels, however, SMCs were unresponsive to infused bFGF. Infusion of a "mitotoxin" (bFGF conjugated to saporin) caused a greater than 50% decrease in the number of viable SMCs in the arterial wall after balloon injury. Prolonged administration of bFGF (12-mu-g/day for 2 weeks) after balloon injury caused an approximately twofold increase in intimal thickening. These results show that bFGF, which is synthesized by the arterial wall, could be a potent mitogen for SMCs in vivo and suggest that any release of endogenous bFGF may be capable of stimulating SMC proliferation, which may subsequently lead to intimal lesion formation.