Differential recognition of lipid domains by two Gb3-binding lectins
Differential recognition of lipid domains by two Gb3-binding lectins
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两种 Gb3 结合凝集素对脂质结构域的差异识别
DOI：
10.1038/s41598-020-66522-8
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发表时间：
2020
影响因子：
4.6
通讯作者：
Römer W
中科院分区：
文献类型：
--
作者：
Schubert T;Sych T;Madl J;Omidvar R;Patalag LJ;Ries A;Kettelhoit K;Brandel A;Mely Y;Steinem C;Werz DB;Thuenauer R;Römer W
来自铜绿假单胞菌的两种凝集素LecA和来自痢疾志贺菌的志贺毒素b亚基（StxB）共享鞘糖脂球三酰神经酰胺（Gb3）作为受体。与我们的直觉相反，我们发现LecA和StxB在识别细胞质膜上的Gb3后分离到不同的结构域。我们假设嵌入脂质双分子层的Gb3碳水化合物头基团的取向不同地影响LecA和StxB的结合。为了验证这一假设，我们利用巨大的单层囊泡重建了凝集素- gb3的相互作用，并且确实能够重建LecA和StxB的分离。Gb3脂肪酸酰基链结构和局部膜环境都调节了LecA和StxB对Gb3的识别。具体来说，与LecA相比，StxB偏爱更有序的膜。基于我们的发现，我们建议比较LecA和StxB的染色模式作为评估细胞膜秩序的替代方法。为了验证这种方法，我们重新建立了上皮细胞的顶端质膜比基底外侧质膜更有序。此外，我们发现StxB在初级纤毛和睫状膜处识别Gb3，而LecA仅结合睫状膜周围的Gb3。这表明睫状体膜比周围的睫状体周膜的阶高。
The two lectins LecA fromPseudomonas aeruginosaand the B-subunit of Shiga toxin fromShigella dysenteriae(StxB) share the glycosphingolipid globotriaosylceramide (Gb3) as receptor. Counterintuitively, we found that LecA and StxB segregated into different domains after recognizing Gb3 at the plasma membrane of cells. We hypothesized that the orientation of the carbohydrate head group of Gb3 embedded in the lipid bilayer differentially influences LecA and StxB binding. To test this hypothesis, we reconstituted lectin-Gb3 interaction using giant unilamellar vesicles and were indeed able to rebuild LecA and StxB segregation. Both, the Gb3 fatty acyl chain structure and the local membrane environment, modulated Gb3 recognition by LecA and StxB. Specifically, StxB preferred more ordered membranes compared to LecA. Based on our findings, we propose comparing staining patterns of LecA and StxB as an alternative method to assess membrane order in cells. To verify this approach, we re-established that the apical plasma membrane of epithelial cells is more ordered than the basolateral plasma membrane. Additionally, we found that StxB recognized Gb3 at the primary cilium and the periciliary membrane, whereas LecA only bound periciliary Gb3. This suggests that the ciliary membrane is of higher order than the surrounding periciliary membrane.
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影响因子：
4.3
作者：
Chen, CP;Song, SC;Wu, AM
通讯作者：
Wu, AM
影响因子：
3.4
作者：
K. Raghunathan;Tiffany Wong;D. Chinnapen;W. Lencer;M. Jobling;A. Kenworthy
通讯作者：
K. Raghunathan;Tiffany Wong;D. Chinnapen;W. Lencer;M. Jobling;A. Kenworthy
影响因子：
3.4
作者：
Sezgin, Erdinc;Levental, Ilya;Schwille, Petra
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Schwille, Petra
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影响因子：
2.8
作者：
D. G. Pina;L. Johannes
通讯作者：
L. Johannes
影响因子：
11.8
作者：
Chinnapen, Daniel J-F.;Hsieh, Wan-Ting;te Welscher, Yvonne M.;Saslowsky, David E.;Kaoutzani, Lydia;Brandsma, Eelke;D'Auria, Ludovic;Park, Hyejung;Wagner, Jessica S.;Drake, Kimberly R.;Kang, Minchul;Benjamin, Thomas;Ullman, M. David;Costello, Catherine E.;Kenworthy, Anne K.;Baumgart, Tobias;Massol, Ramiro H.;Lencer, Wayne I.
通讯作者：
Lencer, Wayne I.