Stretching of single DNA molecules complexed with restriction endonuclease by Langmuir-Blodgett method.
Stretching of single DNA molecules complexed with restriction endonuclease by Langmuir-Blodgett method.
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DOI：
10.1016/j.colsurfb.2004.10.025
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发表时间：
2005-02
期刊：
影响因子：
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通讯作者：
Y. Matsuo;K. Ijiro;M. Shimomura
中科院分区：
文献类型：
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作者：
Y. Matsuo;K. Ijiro;M. Shimomura
我们提出了一种新的技术，拉伸单双链DNA分子在固体基板上的LB方法。将在空气-水界面处形成的阳离子两亲物和DNA分子的聚离子复合物单层转移到干净的玻璃基底上。垂直提升玻璃基底提供了转移的单层，其由平行于玻璃上的提升方向排列的拉伸的单个DNA分子组成。用限制性内切酶（EcoRI）复合的DNA分子，通过使用这种方法进行拉伸。转移的单层的荧光图像显示EcoRI结合的DNA分子可以被拉伸并固定在玻璃基底上。由EcoRI识别的DNA的特定序列被检测为拉伸的DNA分子的空间位置。
We have proposed a new technique for stretching single double-stranded DNA molecules on solid substrates by the Langmuir–Blodgett (LB) method. The polyion complex monolayer of a cationic amphiphile and DNA molecules formed at the air–water interface was transferred on a clean glass substrate. Vertical lifting up of the glass substrate provided the transferred monolayer consisting the stretched individual DNA molecules aligned parallel to the lifting direction on the glass. The DNA molecules complexed with the restriction endonuclease (EcoRI) were employed for stretching by using this method. Fluorescence images of the transferred monolayer showed that the EcoRI-binding DNA molecules could be stretched and immobilized on the glass substrate. A specific sequence of DNA recognized by EcoRI was detected as spatial positions of the stretched DNA molecules.