Resiquimod is a modest adjuvant for HIV-1 gag-based genetic immunization in a mouse model.
Resiquimod is a modest adjuvant for HIV-1 gag-based genetic immunization in a mouse model.
复制标题
Resiquimod 是一种温和的佐剂，用于小鼠模型中基于 HIV-1 gag 的基因免疫。
DOI：
10.1016/j.vaccine.2004.01.037
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发表时间：
2004
期刊：
影响因子：
5.5
通讯作者：
Weiner,DavidB
中科院分区：
文献类型：
--
作者：
Otero,Miguel;Calarota,SandraA;Felber,Barbara;Laddy,Dominic;Pavlakis,George;Boyer,JeanD;Weiner,DavidB
DNA vaccines have been effective at generating useful immune responses in many animal species. However, it is clearly desirable to increase their potency. The identification of adjuvants that increase their cell-mediated immune (CMI) response is therefore an important goal. Resiquimod is an imiquimod analog proven to activate dendritic cells through TLR-7. The adjuvant capacity of resiquimod has not, to our knowledge, been studied in the context of genetic immunization. Here, we studied resiquimod as an adjuvant for plasmid vaccine therapy by intra-muscular immunization of BALB/c mice with HIV-1 gag DNA vaccine without and with several concentrations of resiquimod (ranging from 5–100nM). We observed that resiquimod moderately enhanced IFN-γ production as measured by a peptide-based ELISPOT assay compared to that obtained in mice immunized with DNA gag only. Antigen-specific T-cell proliferation studies showed a several-fold increase in the stimulation index in mice immunized with DNA gag +50 nM of resiquimod as compared to mice receiving DNA gag alone. Antibody titer also increased, while the antibody isotyping data showed a strong Th1 biased type response. Analysis of cytokine production in serum samples demonstrated a stronger Th1 cytokine bias in the presence of resiquimod. Furthermore, relevant increase in IL-4 production, as measured by ELISPOT assay, was not observed. Our results show that resiquimod can have modest adjuvant activity, in a DNA formulation, driving the immune system towards a cell-mediated immune response. Additional studies involving this adjuvant for DNA vaccines are underway.
