Development and characterization of microsatellite markers for Lilium longiflorum (Liliaceae).

Development and characterization of microsatellite markers for Lilium longiflorum (Liliaceae).
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DOI:
10.3732/apps.1300014
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发表时间:
2013-09
影响因子:
3.6
通讯作者:
Okubo H
Okubo H
中科院分区:
生物学4区
文献类型:
--
作者:
Sakazono S;Hiramatsu M;Watanabe M;Okubo H

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·研究报告:利用10对微卫星引物对麝香百合种内遗传分化进行了研究。·方法和结果:利用双重抑制PCR技术分离出61个微卫星位点,其中10个位点可有效地描述和估计两个群体的遗传变异。麝香草在每个位点的等位基因的数量是不同的人口(平均= 3.2和10.3等位基因每个位点),平均观察到的杂合性值分别为0.245和0.732。结论:研究结果表明,不同群体间存在着显著的遗传变异,本研究所开发的微卫星标记将为研究天然野生胡枝子的遗传结构和交配系统提供有用的工具。longiflorum populations.
• Premise of the study: Ten microsatellite primers were developed to obtain information on genetic variation in Lilium longiflorum, a bulbous species showing high intraspecific genetic differentiation. • Methods and Results: Of 61 microsatellite loci isolated using the dual suppression PCR technique, 10 loci were effective to characterize and estimate genetic variation in two populations of L. longiflorum. The number of alleles at each locus was different between the populations (averages = 3.2 and 10.3 alleles per locus), and the mean observed heterozygosity values were 0.245 and 0.732. • Conclusions: Our results demonstrate that there is significant genetic variation between the populations and that the microsatellite markers developed in this study will be useful tools for the investigation of the genetic structure and mating system of natural L. longiflorum populations.
DOI: 10.1046/j.1471-8286.2003.00467.x
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期刊: MOLECULAR ECOLOGY NOTES
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