Microfluidic and hydrothermal preparation of vesicles using sorbitan monolaurate/polyoxyethylene (20) sorbitan monolaurate (Span 20/Tween 20)
Microfluidic and hydrothermal preparation of vesicles using sorbitan monolaurate/polyoxyethylene (20) sorbitan monolaurate (Span 20/Tween 20)
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使用脱水山梨醇单月桂酸酯/聚氧乙烯 (20) 脱水山梨醇单月桂酸酯 (Span 20/Tween 20) 进行微流体和水热制备囊泡
DOI：
10.1016/j.colsurfb.2021.111836
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Kimura Yukitaka
中科院分区：
文献类型：
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作者：
Shimanouchi Toshinori;Hayashi Tetsuya;Toramoto Kazuki;Fukuma Saki;Hayashi Keita;Yasuhara Kazuma;Kimura Yukitaka
在这里，我们提出了一种水热乳化和溶剂扩散(SD)相结合的方法来制备囊泡。由于山梨醇单月桂酸酯/聚氧乙烯(20)山梨醇单月桂酸酯(Span 20/Tween20)体系价格低廉，且生产规模较大，因此选择该体系作为目标脂类。在水热条件下(2 4 0 °C、10 Mpa)形成含脂油包水(W/O)乳状液，然后与含脂类的水混合，得到W/O/W乳状液。作为后续工艺的W/O/W乳液的SD产生了囊泡。最佳制备条件为：50：50 wt%Span 20/Tween20(最终脂肪浓度12 mm)，以辛酸为有机溶剂，水热温度240 °C，反应时间4 min，反应温度4 °C，反应时间24 h。得到的囊泡直径最大为10 0 nm，与SD前的W/O/W乳剂相当。这表明W/O/W乳状液作为囊泡形成的模板。囊泡的数密度、直径和膜性质取决于水/油/脂体系的混合比例。与常规方法制备的囊泡相比，囊泡的数密度较低。
Here, we present a method for preparing vesicles by combining hydrothermal emulsification with solvent diffusion (SD). The sorbitan monolaurate/polyoxyethylene (20) sorbitan monolaurate (Span 20/Tween 20) system was used as the target lipid because these lipids are cheap and advantageous for the production scale. The water-in-oil (W/O) emulsion stabilized with lipids was formed under hydrothermal conditions (240 °C under 10 MPa), followed by mixing with water that included lipids to obtain a W/O-in-water (W/O/W) emulsion. The SD for the W/O/W emulsion as a subsequent process yielded vesicles. The optimal preparation conditions were 50:50 wt% Span 20/Tween 20 as a mixing ratio (final lipid concentration 12 mM), octanoic acid as an organic solvent, 240 °C for 4 min during the hydrothermal treatment, and 4 °C for 24 h in the SD process. The diameter of the vesicles obtained was at most 100 nm, which was comparable to that of the W/O/W emulsion before SD. This suggested that the W/O/W emulsion acted as a template for vesicle formation. The number density, diameter, and membrane properties of vesicles depend on the mixing ratio of the water/oil/lipid system. Specifically, the number density of vesicles was low relative to that of vesicles prepared by the conventional method.