Automatic cell counting with ImageJ
Automatic cell counting with ImageJ
复制标题
DOI：
10.1016/j.ab.2014.12.007
复制
发表时间：
2015-03-15
影响因子：
2.9
通讯作者：
Grishagin, Ivan V.
中科院分区：
文献类型：
--
作者：
Grishagin, Ivan V.
细胞计数是一项重要的常规程序。然而，到目前为止，还没有全面、易用、廉价的常规细胞计数解决方案，这一过程通常需要手动执行。在这里，我们报告了一种自动细胞计数的完整解决方案，其中传统的光学显微镜配备了网络摄像机，以获取血细胞计数仪组件中哺乳动物细胞悬浮的图像。基于ImageJ工具箱，我们设计了两种算法来自动对这些细胞进行计数。与手动计数相比，这种方法的速度大约快了10倍，并产生了更可靠和一致的结果。(C)2014 Elsevier Inc.保留所有权利。
Cell counting is an important routine procedure. However, to date there is no comprehensive, easy to use, and inexpensive solution for routine cell counting, and this procedure usually needs to be performed manually. Here, we report a complete solution for automatic cell counting in which a conventional light microscope is equipped with a web camera to obtain images of a suspension of mammalian cells in a hemocytometer assembly. Based on the ImageJ toolbox, we devised two algorithms to automatically count these cells. This approach is approximately 10 times faster and yields more reliable and consistent results compared with manual counting. (C) 2014 Elsevier Inc. All rights reserved.