Estrogen activates endothelial exocytosis.
Estrogen activates endothelial exocytosis.
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雌激素激活内皮胞吐作用。
DOI：
10.1016/j.bbrc.2021.04.019
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发表时间：
2021-06-18
影响因子：
3.1
通讯作者：
Lowenstein CJ
中科院分区：
文献类型：
--
作者：
Kim CS;Yea K;Morrell CN;Jeong Y;Lowenstein CJ
Estrogen replacement for post-menopausal women has been proven beneficial and widely used in treating post-menopausal symptoms such as hot flush, vaginal dryness and osteoporosis. However, its cardiovascular benefit is still controversial. Here, we show that estrogen elicits endothelial exocytosis, the key step in vascular thrombosis and inflammation. Exogenous 17β-estradiol (E2) stimulated endothelial exocytosis of Weibel-Palade bodies (WPBs), releasing von Willebrand factor (vWF) and interleukin-8 (IL-8). Conversely, the estrogen antagonist ICI-182,780 interfered with E2-induced endothelial exocytosis. The ERα agonist propyl pyrazole triol (PPT), but not the ERβ agonist diarylpropionitrile (DPN), induced vWF release, while ERα silencing counteracted vWF release by E2, suggesting that ERα mediates this effect. Exocytosis triggered by E2 occurred rapidly within 15 minutes and was not inhibited by either actinomycin D or cycloheximide. On the contrary, it was inhibited by the pre-treatment of U0126 or SB203580, an ERK or p38 inhibitor, suggesting that E2-induced endothelial exocytosis is non-genomically mediated by the MAP kinase pathway. Finally, E2 treatment enhanced platelet adhesion to endothelial cells ex vivo, which was interfered with the pre-treatment of ICI-182,780 or U0126. Taken together, our data indicate that estrogen activates endothelial exocytosis non-genomically through the ERα-MAP kinase pathway, leading to vascular thromboembolism. Our data further suggest that potential cardiovascular side effects should be considered for post-menopausal E2 supplement.
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DOI：
10.3390/molecules21080973
发表时间：
2016-07-25
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Hohmann N;Xia N;Steinkamp-Fenske K;Förstermann U;Li H
通讯作者：
Li H
影响因子：
5.5
作者：
Pelekanou, Vasiliki;Kampa, Marilena;Notas, George
通讯作者：
Notas, George
DOI：
10.1161/atvbaha.120.314159
发表时间：
2020-09-01
影响因子：
8.7
作者：
Adlanmerini, Marine;Febrissy, Chanaelle;Lenfant, Francoise
通讯作者：
Lenfant, Francoise
DOI：
10.1006/bbrc.1997.7085
发表时间：
1997-08-18
影响因子：
3.1
作者：
Alvarez, RJ;Gips, SJ;GoldschmidtClermont, PJ
通讯作者：
GoldschmidtClermont, PJ
DOI：
10.1056/nejmoa1505241
发表时间：
2016-03-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Hodis HN;Mack WJ;Henderson VW;Shoupe D;Budoff MJ;Hwang-Levine J;Li Y;Feng M;Dustin L;Kono N;Stanczyk FZ;Selzer RH;Azen SP;ELITE Research Group
通讯作者：
ELITE Research Group