The structure of C-banded human metaphase chromosomes as observed by atomic force microscopy.
The structure of C-banded human metaphase chromosomes as observed by atomic force microscopy.
复制标题
DOI：
10.1679/aohc.68.81
复制
发表时间：
2005
影响因子：
--
通讯作者：
D. Fukushi;T. Ushiki
中科院分区：
文献类型：
--
作者：
D. Fukushi;T. Ushiki
应用光学显微镜和原子力显微镜（AFM）对人类中期染色体C-带的超微结构进行了研究。光镜下C-带染色体显示，除Y染色体外，所有染色体的着丝粒区均呈阳性染色。原子力显微镜进一步显示，C-阳性区域高于C-阴性区域。C-阳性区域的面积是特定的，这取决于每个染色体;它的范围从着丝粒到近端的长臂在1号染色体，而它仅限于着丝粒在2号和3号染色体。在更高的放大倍数下，在染色体的全长中清晰地显示出约50 nm厚的染色质纤维。在C-阳性区，这些染色质纤维密集，而在C-阴性区，染色质纤维松散，轻微扭曲。这些AFM研究结果表明，某些相关的染色质纤维压实的因素仍然在C-阳性区域，即使连续的C-敲打处理。
The ultrastructure of C-banded human metaphase chromosomes was studied by the combined use of light microscopy and atomic force microscopy (AFM). Light microscopy of the C-banded chromosomes showed that the centromeric regions of all chromosomes except the Y chromosome were positively stained. AFM further revealed that the C-positive region was higher than the C-negative region. The area of the C-positive region was specific depending on each chromosome; it ranged from the centromere to the proximal end of the long arm in chromosome 1, while it was restricted to the centromere in chromosomes 2 and 3. At higher magnification, chromatin fibers about 50 nm thick were clearly shown in the entire length of the chromosomes. In the C-positive region, these chromatin fibers were densely packed, while chromatin fibers were loosely packed with gentle twisting in the C-negative region. These AFM findings suggest that certain factors related to the chromatin fiber compaction remain in the C-positive region even after successive C-banging treatment.