An in vivo murine model for screening cranial bone regenerative materials: testing of a novel synthetic collagen gel.
An in vivo murine model for screening cranial bone regenerative materials: testing of a novel synthetic collagen gel.
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用于筛选颅骨再生材料的体内小鼠模型：新型合成胶原凝胶的测试。
DOI：
10.1007/s10856-014-5185-5
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Tsuyoshi Takato.
中科院分区：
文献类型：
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作者：
Hisako Hikiji;Ken Tomizuka;Tetsushi Taguchi;Hiroyuki Koyama;Daichi Chikazu;Yoshiyuki Mori;Tsuyoshi Takato.
需要快速有效的动物模型来评估许多新的候选骨再生材料的有效性。我们开发了一种体内模型筛选脂多糖（LPS）处理的小鼠颅骨骨再生，在20分钟的手术中将材料覆盖在颅骨骨膜上，并在1周内检测到结果。腹腔注射LPS可降低成骨的自发性，局部应用碱性成纤维细胞生长因子（bFGF）可提高成骨细胞的成骨活性。一种新的合成胶原凝胶，碱处理胶原（AlCol）交联柠檬酸三琥珀酰（TSC），作为一个水库的基本物质，如bFGF。AlCol-TSC凝胶与bFGF结合可激活成骨细胞活性，而不会因单独使用bFGF而导致类骨成熟延迟。乙醇- tsc凝胶可以减缓bFGF的释放，改善类骨形成和骨矿化之间的不平衡。这些发现表明，我们的模型适合筛选骨再生材料，并且AlCOl-TSC凝胶可作为bFGF缓释的候选储层。
Rapid and efficient animal models are needed for evaluating the effectiveness of many new candidate bone regenerative materials. We developed an in vivo model screening for calvarial bone regeneration in lipopolysaccharide (LPS)-treated mice, in which materials were overlaid on the periosteum of the calvaria in a 20 min surgery and results were detectable in 1 week. Intraperitoneal LPS injection reduced spontaneous bone formation, and local application of basic fibroblast growth factor (bFGF) increased the bone-forming activities of osteoblasts. A novel synthetic collagen gel, alkali-treated collagen (AlCol) cross-linked with trisuccinimidyl citrate (TSC), acted as a reservoir for basic substances such as bFGF. The AlCol–TSC gel in conjunction with bFGF activated osteoblast activity without the delay in osteoid maturation caused by bFGF administration alone. The AlCol–TSC gel may slow the release of bFGF to improve the imbalance between osteoid formation and bone mineralization. These findings suggest that our model is suitable for screening bone regenerative materials and that the AlCOl–TSC gel functions as a candidate reservoir for the slow release of bFGF.