ZnO nanorods as an intracellular sensor for pH measurements
ZnO nanorods as an intracellular sensor for pH measurements
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DOI：
10.1063/1.2798582
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发表时间：
2007-10-15
影响因子：
3.2
通讯作者：
Stralfors, Peter
中科院分区：
文献类型：
--
作者：
Al-Hilli, Safaa M.;Willander, M.;Stralfors, Peter
ZnO纳米棒的直径为80 nm，长度为700 nm，生长在硼硅酸盐玻璃毛细管（直径为0.7 μ m）的尖端上，用于创建一个高度灵敏的pH传感器，用于监测单细胞内的体内生物过程。ZnO纳米棒，功能化质子H3 O+和羟基OH-基团，表现出pH依赖的电化学电位差与Ag/AgCl微电极。电位差在很大的动态范围（4-11）内呈线性，这可以从质子化和去质子化过程中表面电荷的变化来理解。这些纳米电极装置具有在单个活细胞中进行分析测量的能力，并且具有感测细胞内特定位置中的单个化学物质的能力。
ZnO nanorods with 80 nm diameter and 700 nm length and grown on the tip of a borosilicate glass capillary (0.7 mu m in diameter) were used to create a highly sensitive pH sensor for monitoring in vivo biological process within single cells. The ZnO nanorods, functionalized by proton H3O+ and hydroxyl OH- groups, exhibit a pH-dependent electrochemical potential difference versus a Ag/AgCl microelectrode. The potential difference was linear over a large dynamic range (4-11), which could be understood in terms of the change in surface charge during protonation and deprotonation. These nanoelectrode devices have the ability to enable analytical measurements in single living cells and have the capability to sense individual chemical species in specific locations within a cell.