Synthesis of RNA oligomers on heterogeneous templates
Synthesis of RNA oligomers on heterogeneous templates
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DOI：
10.1038/379238a0
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发表时间：
1996-01-18
期刊：
影响因子：
64.8
通讯作者：
Ferris, JP
中科院分区：
文献类型：
--
作者：
Ertem, G;Ferris, JP
在生命的化学起源中，RNA世界（1，2）的概念很有吸引力，因为核酸既能存储信息，又能充当催化互补分子合成的模板（3）。模板指导的合成已被证明用于同质寡核苷酸，其与天然核酸一样，在核苷酸单体之间具有3 '，5'键（4-7）。但似乎益生元途径RNA样分子将产生异质分子，与各种磷酸二酯键和线性和环状核苷酸链。在这里，我们表明，这种异质性不需要是互补分子的模板化的障碍。具体而言，我们表明，异质oligocytidylates，由蒙脱石粘土催化的缩合致动单体，可以作为模板的oligoguanylates的合成。此外，我们表明，寡胞苷酸是专门的2 '，5'-连接也可以直接合成的寡鸟苷酸。这种异质模板反应可能增加了生命最终从中出现的原核酸库的多样性。
THE concept of an RNA world(1,2) in the chemical origin of life is appealing, as nucleic acids are capable of both information storage and acting as templates that catalyse the synthesis of complementary molecules(3). Template-directed synthesis has been demonstrated for homogeneous oligonucleotides that, like natural nucleic acids, have 3',5' linkages between the nucleotide monomers(4-7). But it seems likely that prebiotic routes to RNA-like molecules would have produced heterogeneous molecules,vith various kinds of phosphodiester linkages and both linear and cyclic nucleotide chains. Here we show that such heterogeneity need be no obstacle to the templating of complementary molecules. Specifically, we show that heterogeneous oligocytidylates, formed by the montmorillonite clay-catalysed condensation of actuated monomers, can serve as templates for the synthesis of oligoguanylates. Furthermore, we show that oligocytidylates that are exclusively 2',5'-linked can also direct synthesis of oligoguanylates. Such heterogeneous templating reactions could have increased the diversity of the pool of protonucleic acids from which life ultimately emerged.