Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.).
Genome-wide analysis and characterization of Aux/IAA family genes related to fruit ripening in papaya (Carica papaya L.).
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DOI：
10.1186/s12864-017-3722-6
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发表时间：
2017-05-05
期刊：
影响因子：
4.4
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Liu K;Yuan C;Feng S;Zhong S;Li H;Zhong J;Shen C;Liu J
生长素/吲哚-3-乙酸（Aux/IAA）家族基因编码短寿命的核蛋白，其介导生长素相关基因的反应，并参与多种植物发育和生长过程。然而，Aux/IAA基因在番木瓜果实发育和成熟过程中的作用尚不清楚。基本上是未知的。本研究对18株C.番木瓜Aux/IAA（CpIAA）基因的研究。木瓜中的Aux/IAA基因家族比拟南芥中的略小，但所有的系统发育亚家族都被代表。大多数CpIAA基因对各种植物激素有反应，并以组织特异性方式表达。为了解CpIAA基因在果实发育和成熟过程中的生物学功能，采用实时荧光定量PCR技术分析了CpIAA基因在不同发育阶段的表达谱。此外，IAA处理显着延迟成熟过程中的番木瓜果实在早期阶段。ACC和1-MCP处理后CpIAA基因表达的变化表明，番木瓜果实成熟过程中存在乙烯和生长素的交互作用。本研究为番木瓜Aux/IAA家族的基因结构、系统发育关系和表达谱的研究提供了全面的信息。CpIAA基因在果实发育和成熟过程中的表达变化为我们了解生长素信号在番木瓜生殖器官成熟中的作用提供了机会。本文的在线版本（doi：10.1186/s12864-017-3722-6）包含补充材料，可供授权用户使用。
Auxin/indole-3-acetic acid (Aux/IAA) family genes encode short-lived nuclear proteins that mediate the responses of auxin-related genes and are involved in several plant developmental and growth processes. However, how Aux/IAA genes function in the fruit development and ripening of papaya (Carica papaya L.) is largely unknown. In this study, a comprehensive identification and a distinctive expression analysis of 18 C. papaya Aux/IAA (CpIAA) genes were performed using newly updated papaya reference genome data. The Aux/IAA gene family in papaya is slightly smaller than that in Arabidopsis, but all of the phylogenetic subfamilies are represented. Most of the CpIAA genes are responsive to various phytohormones and expressed in a tissues-specific manner. To understand the putative biological functions of the CpIAA genes involved in fruit development and ripening, quantitative real-time PCR was used to test the expression profiling of CpIAA genes at different stages. Furthermore, an IAA treatment significantly delayed the ripening process in papaya fruit at the early stages. The expression changes of CpIAA genes in ACC and 1-MCP treatments suggested a crosstalk between auxin and ethylene during the fruit ripening process of papaya. Our study provided comprehensive information on the Aux/IAA family in papaya, including gene structures, phylogenetic relationships and expression profiles. The involvement of CpIAA gene expression changes in fruit development and ripening gives us an opportunity to understand the roles of auxin signaling in the maturation of papaya reproductive organs. The online version of this article (doi:10.1186/s12864-017-3722-6) contains supplementary material, which is available to authorized users.