Identification of a new expanding family of genes characterized by atypical LRR domains.: Localization of a cluster preferentially expressed in oocyte
Identification of a new expanding family of genes characterized by atypical LRR domains.: Localization of a cluster preferentially expressed in oocyte
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DOI：
10.1016/s0014-5793(03)01341-3
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发表时间：
2003-12-18
期刊：
影响因子：
3.5
通讯作者：
Monget, P
中科院分区：
文献类型：
--
作者：
Dadé, S;Callebaut, I;Monget, P
在目前的工作中，我们已经使用了计算机减法方法来确定新的卵母细胞特异性基因在小鼠。通过这种方式，我们已经在计算机上确定了一个由80多个成员组成的新基因家族。序列分析表明，这些基因属于富含亮氨酸重复序列（LRR）蛋白超家族。然而，该家族的LRR在长度和β链区域内的氨基酸组成方面显示出一些变异性，因为与典型LRR相比，更多的亮氨酸残基被其他疏水性氨基酸取代。有趣的是，其中9个基因的EST几乎只存在于小鼠卵子文库中。其中三个被选中进行实验研究。通过RT-PCR和原位杂交，我们证实了它们在小鼠从初级卵泡到排卵前卵泡的卵母细胞中的特异性表达。这三个基因位于小鼠4号染色体上的一个簇中，靠近另一个最近发现的卵母细胞特异性基因，称为卵生成素，我们也发现它属于同一家族。因此，我们将后者的基因重新命名为'oogenesin-1'，并在这里确定的三个基因被命名为oogenesin-2，-3和-4。(C)2003年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
In the present work, we have used the in silico subtraction methodology to identify novel oocyte-specific genes in the mouse. By this way, we have identified in silico a new family of genes composed of more than 80 members. Sequence analysis showed that these genes belong to the superfamily of leucine-rich repeat (LRR) proteins. However, LRRs of this family display some variability in length and in amino acids composition within the beta-strands region, as more leucine residues are substituted by other hydrophobic amino acids as compared to canonical LRRs. Interestingly, for nine of these genes, the ESTs were represented almost exclusively in mouse egg libraries. Three of them were selected for experimental study. By RTPCR and in situ hybridization, we confirmed their specific expression in the mouse oocyte from primary to preovulatory follicles. These three genes are localized in a cluster on mouse chromosome 4, in the vicinity of another recently discovered oocyte specific gene called oogenesin, that we also found to belong to the same family. We thus re-named this latter gene 'oogenesin-1', and the three genes identified here were named oogenesin-2, -3 and -4. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.