Off-line capillary electrophoresis/fully automated nanoelectrospray chip quadrupole time-of-flight mass spectrometry and tandem mass spectrometry for glycoconjugate analysis
Off-line capillary electrophoresis/fully automated nanoelectrospray chip quadrupole time-of-flight mass spectrometry and tandem mass spectrometry for glycoconjugate analysis
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DOI：
10.1002/jms.707
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发表时间：
2004-10-01
影响因子：
2.3
通讯作者：
Zamfir, A
中科院分区：
文献类型：
--
作者：
Bindila, L;Almeida, R;Zamfir, A
描述了离线毛细管电泳 (CE)/(-)nanoESIchip-四极杆飞行时间 (QTOF) 质谱 (MS) 和串联 MS 系统的实施和优化，用于复杂碳水化合物混合物中组分的成分图谱和结构研究。该方法是为糖筛选而开发的，并应用于患有 α-N-乙酰己糖胺酶缺乏症（称为辛德勒病）的患者尿液中的 O-糖基化肽。灵敏度的基本问题以前是离线 CE/MS 分析的一个严重缺陷，现在可以通过 CE 与基于自动化芯片的 ESI-QTOF-MS 的离线结合来积极解决，从而为 CE/芯片 MS 耦合提供灵活性，并增强异质混合物中单一组分的结构阐明。版权所有 (C) 2004 John Wiley Sons, Ltd.
Implementation and optimization of an off-line capillary electrophoresis (CE)/(-)nanoESIchip-quadrupole time-of-flight (QTOF) mass spectrometric (MS) and tandem MS system for compositional mapping and structural investigation of components in complex carbohydrate mixtures is described. The approach was developed for glycoscreening and applied to O-glycosylated peptides from urine of a patient suffering from alpha-N-acetylhexosaminidase deficiency, known as Schindler's disease. The fundamental issue of sensitivity, previously representing a serious drawback of the off-line CE/MS analysis, could be positively addressed by the off-line conjunction of CE with automated chip-based ESI-QTOF-MS to provide flexibility for CE/chip MS coupling and enhance structural elucidation of single components in heterogeneous mixtures. Copyright (C) 2004 John Wiley Sons, Ltd.