Immunoreactivity of two novel monoclonal antibodies against human inducible co-stimulator ligand.
Immunoreactivity of two novel monoclonal antibodies against human inducible co-stimulator ligand.
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两种新型单克隆抗体针对人诱导共刺激剂配体的免疫反应性。
DOI：
10.1089/hyb.2011.0014
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Xueguang Zhang
中科院分区：
文献类型：
--
作者：
S. Shen;Fengming Wang;Liwen Chen;Ting Wang;Yu;Xueguang Zhang
人诱导型共刺激分子配体（GL 50，CD 275），又称B7-H2或ICOSL，是B7/CD 28超家族的一种阳性共刺激分子，在免疫应答中起着重要作用。在这里，我们使用经典的杂交瘤技术产生了两个新的小鼠抗人GL 50单克隆抗体（MAbs）。两种单克隆抗体（克隆2B 4和4D 11）分别为IgG 1（κ）和IgG 2a（κ），并与人GL 50特异性结合。表位竞争试验表明，2B 4和4D 11与GL 50的相同表位结合，这不被市售GL 50单抗（9 F-8A 4）识别。在功能上，这两种MAb充当T细胞增殖的阻断剂。这两种抗体作为一种有用的工具，对进一步研究GL 50的免疫识别和功能具有重要的价值。
Human inducible co-stimulator ligand (GL50, CD275), also known as B7-H2 or ICOSL, is a positive co-stimulatory molecule of B7/CD28 superfamily, which plays a critical role in immune response. Here we generated two novel mouse anti-human GL50 monoclonal antibodies (MAbs) using classical hybridoma technology. The two MAbs (clones 2B4 and 4D11) were IgG1 (κ) and IgG2a (κ), respectively, and bound specifically to human GL50. Epitope competition assay showed that 2B4 and 4D11 bind to the same epitope of GL50, which is not recognized by the commercially available GL50 MAb (9F-8A4). Functionally, the two MAbs act as a blocker of T cell proliferation. Taken together, as useful tools, these two antibodies might be of great value for further exploration of the immune identification and function of GL50.
影响因子：
8.6
作者：
R. Sporici;Richard L. Beswick;C. Allmen;C. A. Rumbley;M. Hayden-Ledbetter;J. Ledbetter;P. Perrin
通讯作者：
R. Sporici;Richard L. Beswick;C. Allmen;C. A. Rumbley;M. Hayden-Ledbetter;J. Ledbetter;P. Perrin
影响因子：
32.4
作者：
Swallow, MM;Wallin, JJ;Sha, WC
通讯作者：
Sha, WC