Effects of dexmedetomidine on activating intestinal glial cells
Effects of dexmedetomidine on activating intestinal glial cells
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右美托咪定对激活肠胶质细胞的作用
DOI：
10.32383/appdr/137967
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发表时间：
2021-08
影响因子：
0.4
通讯作者：
Ma Hebing
中科院分区：
文献类型：
--
作者：
Meng Jun;Zhao Youhui;Yang Jingyuan;Yang Kun;Wu Yi;Ma Hebing
本研究以胶质细胞源性神经营养因子GDNF为评价指标，探讨临床常用麻醉镇静药右美托咪定对肠神经胶质细胞活化的影响。选择大鼠肠神经胶质细胞进行体外实验，用不同浓度的右美托咪定处理细胞24 h。免疫荧光法检测胶质细胞酸性蛋白（GFAP）的表达。RT-qPCR和Western Blot分别检测GDNF的mRNA和蛋白水平。GFAP免疫荧光结果显示，肠神经胶质细胞具有细长的突触，分布于胞体边缘。在右旋美托咪啶处理的细胞和未处理的细胞之间未观察到GAFP表达的差异。然而，右旋美托咪啶处理在mRNA和蛋白水平上显著增加肠胶质细胞中GDNF的表达。右美托咪定可激活肠神经胶质细胞，增加胶质细胞源性神经营养因子（GDNF）的分泌。
: In this study, glial cell-derived neurotrophic factor GDNF was used as an evaluation index to explore the effect of dexmedetomidine, a commonly used clinical anesthetic and sedative, on the activation of intestinal glial cells. Rat intestinal glial cells were selected for in vitro experiments, cells were treated with dexmedetomidine at various concentrations for 24 h. The expression of the glial fibrillary acidic protein (GFAP) was determined by immunofluorescence assay. The mRNA and protein levels of GDNF were measured by RT-qPCR and Western Blot, respectively. Immunofluorescence of GFAP re sults indicated that intestinal glial cells had slender synapses distributed on the edge of the cell body. No differences in GAFP expression were observed between dexmedetomidine treated and untreated cells. However, dexmedetomidine treatment increased the GDNF expression significantly at both mRNA and protein levels in intestinal glial cells. Dexmedetomidine can activate intestinal glial cells and increase the secretion of glial cell-derived neurotrophic factor (GDNF).