Identification and molecular mapping of loci controlling fruit ripening time in tomato
Identification and molecular mapping of loci controlling fruit ripening time in tomato
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DOI：
10.1007/s001220050033
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发表时间：
2000-01-01
影响因子：
5.4
通讯作者：
Mutschler, MA
中科院分区：
文献类型：
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作者：
Doganlar, S;Tanksley, SD;Mutschler, MA
使用 RAPD 标记分析，在番茄“E6203”（正常成熟）和番茄“早熟”（早熟）杂交的 F-2 群体中，鉴定并绘制了由于果实成熟时间缩短（从开花到成熟的天数 = DTR）而与早熟相关的两个数量性状位点 (QTL)。 5 号染色体上的一个 QTL 与成熟时间（5 天）和果实重量（29.3%）的缩短相关，并分别解释了 DTR 和果实重量总表型变异的 15.8% 和 13%。另一个 QTL 位于 12 号染色体上，主要与成熟时间（7 天）的缩短有关，并解释了 DTR 总表型变异的 12.3%。发现该 QTL 的基因作用是部分显性的（d/a=0.41）。这两个 QTL 共同解释了 DTR 总表型变异的 25.1%。此外，在同一 F-2 群体中鉴定出两个与果实重量相关的 QTL，并分别定位到 4 号和 6 号染色体。这两个 QTL 共同解释了果实重量总表型变异的 30.9%。对于所有 QTL，“早樱桃”等位基因都会导致成熟时间和果实重量减少。最显着的 RAPD 标记 (OPAB-06) 的多态性条带与 12 号染色体上成熟时间缩短的 QTL 相关，被转化为裂解扩增多态性 (CAP) 测定，用于标记辅助选择和进一步将早熟时间 (DTR) 基因渗入栽培番茄中。
Using RAPD marker analysis, two quantitative trait loci (QTLs) associated with earliness due to reduced fruit-ripening time (days from anthesis to ripening = DTR) were identified and mapped in an F-2 population derived from a cross between Lycopersicon esculentum 'E6203' (normal ripening) and Lycopersicon esculentum 'Early Cherry' (early ripening). One QTL, on chromosome 5, was associated with a reduction in both ripening time (5 days) and fruit weight (29.3%) and explained 15.8 and 13% of the total phenotypic variation for DTR and fruit weight, respectively The other QTL, on chromosome 12, was primarily associated with a reduction only in ripening time (7 days) and explained 12.3% of the total phenotypic variation for DTR. The gene action at this QTL was found to be partially dominant (d/a=0.41). Together, these two QTLs explained 25.1% of the total phenotypic variation for DTR. Additionally, two QTLs associated with fruit weight were identified in the same F-2 population and mapped to chromosomes 4 and 6, respectively. Together, these two QTLs explained 30.9% of the total phenotype variation for fruit weight. For all QTLs, the 'Early Cherry' alleles caused reductions in both ripening time and fruit weight. The poly morphic band for the most significant RAPD marker (OPAB-06), linked to the reduced ripening time QTL on chromosome 12, was converted to a cleaved amplified polymorphism (CAP) assay for marker-aided selection and further introgression of early ripening time (DTR) into cultivated tomato.