Differences in heat tolerance within a Daphnia magna population: the significance of body PUFA content

Differences in heat tolerance within a Daphnia magna population: the significance of body PUFA content
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大型溞种群内耐热性的差异:体内多不饱和脂肪酸含量的重要性

DOI:
10.1007/s10750-018-3769-7
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发表时间:
2019
期刊:
影响因子:
2.6
通讯作者:
von Elert
von Elert
中科院分区:
生物学3区
文献类型:
--
作者:
Werner;von Elert

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在这项研究中,我们使用的模式organismDaphnia magnato确定在自然种群的耐热性的变化,并将其与身体脂肪酸含量和组成的变化。作为耐热性的代理,我们测量的时间固定(Timm)为25个克隆谱系从一个单一的湖泊。克隆谱系在15°C下在莱茵衣藻上生长。在沉积第一窝时,测定Timmand体脂肪酸。结果表明,不同品种的D. Timm与总PUFA含量呈负相关,表明增加的体PUFA含量可能对高温下的膜性能有害。幼年体细胞生长在克隆谱系之间变化显著,并且与全身PUFA含量无关,因此身体PUFA含量不是PUFA限制的代理。没有相关的证据表明身体EPA含量对Timmor幼鱼生长的影响。我们推测,所观察到的体内PUFA含量的种群内变异可能与昼夜垂直迁移的幅度不同有关。这些不同的幅度意味着不同的克隆谱系之间的昼夜温度波动的程度。
In this study, we use the model organismDaphnia magnato determine variation in heat tolerance within a natural population and relate this to variation in body fatty acid content and composition. As a proxy for heat tolerance we measured time to immobilization (Timm) for 25 clonal lineages isolated from a single lake. The clonal lineages were grown onChlamydomonas reinhardiiat 15°C. Upon deposition of the first clutch,Timmand body fatty acids were determined. We report significant differences ofTimmand of the content of PUFAs and of eicosapentaenoic acid (EPA) among theD. magnaclonal lineages.Timmwas negatively related to total PUFA-content, indicating that increased body PUFA-content may be detrimental for membrane properties at high temperatures. Juvenile somatic growth varied significantly among clonal lineages, and was not related to total body PUFA-content, hence body PUFA-content was no proxy for PUFA-limitation. No correlative evidence for an impact of body EPA-content onTimmor juvenile growth was obtained. We hypothesize that the observed intra-population variability of body PUFA-content might be related to potentially different amplitudes in diel vertical migration in response to fish. These different amplitudes would mean differences in the extent of diurnal temperature fluctuations among the clonal lineages.
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