Down-regulation of vimentin expression inhibits carcinoma cell migration and adhesion
Down-regulation of vimentin expression inhibits carcinoma cell migration and adhesion
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DOI：
10.1016/j.bbrc.2007.06.036
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发表时间：
2007-08-17
影响因子：
3.1
通讯作者：
Maeaettae, Arto
中科院分区：
文献类型：
--
作者：
McInroy, Lorna;Maeaettae, Arto
波形蛋白是一种III型中间丝蛋白，在上皮性癌中表达频繁，与侵袭性和预后不良相关。我们分析了一组癌细胞系在胶原基质上的迁移和黏附。Vimentin阳性的SW480结肠癌细胞和MDA-MB-231乳腺癌细胞的体外侵袭力最高，并通过RNA干扰研究了Vimentin在这些细胞系中的作用。Vimentin表达下调导致在划痕实验和侵袭实验中通过包被胶原胶的细胞培养插入物的迁移受损。在两种细胞系中都观察到了迁移的减退，而细胞粘附性分析显示，在MDA-MB-231细胞中，与纤维胶原的粘附力减弱，而同时表达波形蛋白和角蛋白中间丝的SW480细胞的粘附性没有受到影响。总之，阻断Vimentin的表达抑制了结肠癌和乳腺癌细胞系的迁移和侵袭。(C)2007 Elsevier Inc.保留所有权利。
Vimentin is a type III Intermediate filament protein that is expressed frequently in epithelial carcinomas correlating with invasiveness and poor prognosis. We have analysed migration and adhesion to collagenous matrix of a panel of carcinoma cell lines. In vitro invasiveness was highest in vimentin-positive SW480 colon cancer and MDA-MB-231 breast cancer cells and the role of vimentin in these cell lines was investigated by RNA interference. Down-regulation of vimentin expression resulted in impaired migration in both scratch-wound experiments and in invasion assays through cell culture inserts coated with collagen gel. Compromised migration was observed in both cell lines, whereas cell attachment assays revealed impaired adhesion to fibrillar collagen in MDA-MB-231 cells while the adhesion of vimentin-ablated SW480 cells, that express both vimentin and keratin intermediate filaments was not affected. In conclusion, ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. (c) 2007 Elsevier Inc. All rights reserved.