The bHLH transcription factor PPLS1 regulates the color of pulvinus and leaf sheath in foxtail millet (Setaria italica)
The bHLH transcription factor PPLS1 regulates the color of pulvinus and leaf sheath in foxtail millet (Setaria italica)
复制标题
bHLH 转录因子 PPLS1 调节谷子 (Setaria italica) 的叶枕和叶鞘的颜色
DOI：
10.1007/s00122-020-03566-4
复制
发表时间：
2020-06-01
影响因子：
5.4
通讯作者：
Li, Dayong
中科院分区：
文献类型：
--
作者：
Bai, Hui;Song, Zhenjun;Li, Dayong
Key messageThe bHLH transcription factor, PPLS1, interacts with SiMYB85 to control the color of pulvinus and leaf sheath by regulating anthocyanin biosynthesis in foxtail millet (Setaria italica).AbstractFoxtail millet (Setaria italica), a self-pollinated crop with numerous small florets, is difficult for cross-pollination. The color of pulvinus and leaf sheath with purple being dominant to green is an indicative character and often used for screening authentic hybrids in foxtail millet crossing. Deciphering molecular mechanism controlling this trait would greatly facilitate genetic improvement of cultivars in foxtail millet. Here, using the F2bulk specific-locus amplified fragment sequencing approach, we mapped the putative causal gene for the purple color of pulvinus and leaf sheath (PPLS) trait to a 100 Kb region on chromosome 7. Expression analyses of the 15 genes in this region revealed thatSeita.7G195400(renamed here asPPLS1) was differentially expressed between purple and green cultivars.PPLS1encodes a bHLH transcription factor and is localized in the nucleus with a transactivation activity. Furthermore, we observed that expression of a MYB transcription factor gene,SiMYB85(Seita.4G086300) involved in anthocyanin biosynthesis, shows a totally positive association with that ofPPLS1. Heterologous co-expression of bothPPLS1andSiMYB85in tobacco leaves led to elevated anthocyanin accumulation and expression of some anthocyanin-related genes. Furthermore, PPLS1 physically interacts with SiMYB85. Taken together, our results suggest that PPLS1 interacts with SiMYB85 to control the color of pulvinus and leaf sheath by regulating anthocyanin biosynthesis in foxtail millet.
