CSF cytokine and chemokine profiles in acute disseminated encephalomyelitis
CSF cytokine and chemokine profiles in acute disseminated encephalomyelitis
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DOI：
10.1016/j.jneuroim.2006.03.020
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发表时间：
2006-06-01
影响因子：
3.3
通讯作者：
Kira, Jun-ichi
中科院分区：
文献类型：
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作者：
Ishizu, Takaaki;Minohara, Motozumi;Kira, Jun-ichi
我们同时测定了16种细胞因子/趋化因子在脑脊液（CSF）从14例急性播散性脑脊髓炎（ADEM）和20个对照使用荧光珠为基础的免疫分析。各种细胞因子如IL-1、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IFN-γ、TNF-α、G-CSF和MIP-1 β在ADEM中显著升高。特别是，与对照组平均值相比，G-CSF显示出显著的38倍增加。IFN-γ、IL-6和IL-8与CSF中的炎症参数（如细胞计数和蛋白水平）显著正相关。相反，活化的CD 4（+）记忆T细胞产生的IL-17没有增加。结果提示，ADEM患者脑脊液中与巨噬细胞/小胶质细胞及Th-1、Th-2细胞活化相关的多种细胞因子表达上调。(c)2006 Elsevier B. V.保留所有权利。
We simultaneously measured 16 cytokines/chemokines in cerebrospinal fluid (CSF) from 14 patients with acute disseminated encephalomyelitis (ADEM) and 20 controls using a fluorescent bead-based immunoassay. A variety of cytokines, such as IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN-gamma, TNF-alpha, G-CSF and MIP-1 beta, were significantly elevated in ADEM. In particular, G-CSF showed a marked 38-fold increase compared to the control mean. Significant positive correlations with inflammatory parameters in CSF, such as cell counts and protein levels, were found for IFN-gamma, IL-6 and IL-8. In contrast, IL-17 produced by activated CD4(+) memory T cells was not increased. The results suggested that various cytokines related to activation of macrophages/microglias and Th-1 and Th-2 cells are upregulated in CSF in ADEM. (c) 2006 Elsevier B.V. All rights reserved.