Genomic insights into a robust gamma-aminobutyric acid-producer Lactobacillus brevis CD0817
Genomic insights into a robust gamma-aminobutyric acid-producer Lactobacillus brevis CD0817
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对强大的 γ-氨基丁酸生产者短乳杆菌 CD0817 的基因组见解
DOI：
10.1186/s13568-019-0799-0
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发表时间：
2019-05-24
期刊：
影响因子：
3.7
通讯作者：
Li, Haixing
中科院分区：
文献类型：
--
作者：
Gao, Dandan;Chang, Kunpeng;Li, Haixing
短链乳杆菌CD0817是从健康成人肠道中分离出来的菌株，是目前生产γ-氨基丁酸的最有效的乳酸菌细胞工厂。本研究测定了CD0817的全基因组序列，并与一些相关的短链球菌基因组进行了比较。CD0817基因组由一个2,990,570个碱基的染色体和4个质粒组成。比较基因组学和系统发育分析表明，短链乳杆菌CD0817与低产GABA的短链乳杆菌不是很保守。一个显著的差异是CD0817只含有gadCA操纵子，而低产GABA的短链杆菌含有操纵子和gadB。GadB似乎对GABA的积累只有很小的贡献。CD0817的高GABA产生能力可能与其非凡的基因组有关。
Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of one 2,990,570-bp chromosome and four plasmids. The comparative genomic and phylogenetic analysis revealed that L. brevis CD0817 was not very conserved with low GABA-producing L. brevis strains. A significant divergence was that CD0817 harbors only the gadCA operon whereas the low GABA-producing L. brevis strains contain the operon and gadB. The gadB seemed to only marginally contribute to the accumulation of GABA. The high GABA production ability of CD0817 may be associated with its extraordinary genome.