Near-field scanning optical microscopy for high-resolution membrane studies.
Near-field scanning optical microscopy for high-resolution membrane studies.
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DOI：
10.1007/978-1-62703-137-0_21
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Dunn, Robert C
中科院分区：
文献类型：
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作者：
Huckabay, Heath A;Armendariz, Kevin P;Newhart, William H;Wildgen, Sarah M;Dunn, Robert C
在生物结构存在的长度尺度上直接探测生物结构的愿望推动了各种高分辨率显微镜技术的稳步发展。其中，光学显微镜，特别是基于荧光的方法继续占据主导地位，在生物学研究中，由于其有利的属性。荧光显微镜既灵敏又特异，通常对生物样品是无创的，对于动态研究具有优异的时间分辨率，并且相对便宜。基于光的显微镜还可以利用基于光谱特征、能量转移、偏振和寿命的无数对比机制，以进一步增强测量的特异性或信息内容。然而，历史上，由于光的衍射，空间分辨率被限制在波长的大约一半。近场扫描光学显微镜（NSOM）是目前正在开发的几种光学方法之一，它将荧光显微镜的有利属性与上级空间分辨率相结合。NSOM是特别适合于研究模型和生物膜和应用这些系统进行了讨论。
The desire to directly probe biological structures on the length scales that they exist has driven the steady development of various high-resolution microscopy techniques. Among these, optical microscopy and, in particular, fluorescence-based approaches continue to occupy dominant roles in biological studies given their favorable attributes. Fluorescence microscopy is both sensitive and specific, is generally noninvasive toward biological samples, has excellent temporal resolution for dynamic studies, and is relatively inexpensive. Light-based microscopies can also exploit a myriad of contrast mechanisms based on spectroscopic signatures, energy transfer, polarization, and lifetimes to further enhance the specificity or information content of a measurement. Historically, however, spatial resolution has been limited to approximately half the wavelength due to the diffraction of light. Near-field scanning optical microscopy (NSOM) is one of several optical approaches currently being developed that combines the favorable attributes of fluorescence microscopy with superior spatial resolution. NSOM is particularly well suited for studies of both model and biological membranes and application to these systems is discussed.