Decay of mammalian hexokinase: characterization of the specific proteolytic activity.
Decay of mammalian hexokinase: characterization of the specific proteolytic activity.
复制标题
哺乳动物己糖激酶的衰变：特定蛋白水解活性的表征。
DOI：
10.1016/0167-4838(91)90115-g
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Piomelli,S
中科院分区：
文献类型：
--
作者：
Murakami,K;Piomelli,S
己糖激酶降解活性(HKDA)与己糖激酶在整个纯化过程中是相关的。它不依赖泛素。在葡萄糖存在的情况下，HKDA需要镁ATP。它由葡萄糖的去除(或依赖于ATP的消耗)触发，并在葡萄糖恢复时被屏蔽。PCMB、TLCK和TPCK抑制己糖激酶，激活HKDA。葡萄糖的去除和活性部位的阻断似乎诱发了HKDA。
Hexokinase-degrading activity (HKDA) is associated with hexokinase throughout purification. It is not ubiquitin-dependent. With glucose present, HKDA requires MgATP. It is triggered by removal (or ATP-dependent consumption) of glucose, and masked when glucose is restored. PCMB, TLCK and TPCK suppress hexokinase and activate HKDA. Glucose removal and active-site blockage appear to induce HKDA.