Detection of the Presence of Bartonella henselae in Cats in Istanbul
Detection of the Presence of Bartonella henselae in Cats in Istanbul
复制标题
在伊斯坦布尔检测猫体内是否存在汉赛巴尔通体
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
A. Ilgaz
中科院分区：
文献类型：
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作者：
B. D. Sigirci;A. Ilgaz
在本研究中，确定在伊斯坦布尔的96只宠物，收容所和流浪猫的汉赛巴尔通体的存在。采用两种不同的细菌培养方法对猫的样本进行检查。将采集到儿科裂解隔离管中的血样离心，并将沉淀接种到补充有5%兔血的心浸液琼脂上。用样品接种BACTEC Peds Plus/F，并在BACTEC 9050自动血液培养系统中孵育。培养结束后，根据殖民地形态、生长时间、革兰氏特性和生化特性对菌株进行鉴定，确定固体培养基分离的27株、BACTEC分离的10株为巴尔通体。利用16 S-23 SrRNA基因间区的特异性引物进行PCR扩增，鉴定为汉赛巴尔通体（Bartonella henselae）。总之，汉赛巴尔通体菌血症的患病率在伊斯坦布尔的猫中为28.1%。
In the present study, the determination of the presence of Bartonella henselae in Istanbul in 96 pet, shelter and stray cats were aimed. The samples from the cats were examined by two different bacteriologic culture methods. The blood samples collected into pediatric lysis isolator tubes were spun and the pellet inoculated onto Heart Infusion Agar supplemented with 5% rabbit blood. BACTEC Peds Plus/F were inoculated with samples and incubated in the BACTEC 9050 automated blood culture systems. After the incubation time, growth were evaluated according to the colony morphology, growth time, gram properties and biochemical properties, and it was determined that 27 isolates by solid medium, 10 isolates by BACTEC were Bartonella spp. Isolates were determined as Bartonella henselae by PCR amplification using specific primers of a portion of 16S-23S rRNA intergenic region. In conclusion, the prevalence of Bartonella henselae bacteremia was found 28.1% of cats in Istanbul.
影响因子：
158.5
作者：
KOEHLER, JE;QUINN, FD;TAPPERO, JW
通讯作者：
TAPPERO, JW
影响因子：
158.5
作者：
Koehler, JE;Sanchez, MA;Tappero, JW
通讯作者：
Tappero, JW