Effects of anterolateral and posterolateral cuts around the medial hypothalamus on the immunoreactive ACTH and beta-endorphin levels in selected brain regions of the rat
Effects of anterolateral and posterolateral cuts around the medial hypothalamus on the immunoreactive ACTH and beta-endorphin levels in selected brain regions of the rat
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DOI：
10.1016/s0361-9230(96)00298-5
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发表时间：
1997-01-01
影响因子：
3.8
通讯作者：
Makara, GB
中科院分区：
文献类型：
--
作者：
Barna, I;Koenig, JI;Makara, GB
为了评估在前或后方向离开内侧下丘脑（MH）的ACTH能和β-内啡肽能通路的相对重量，在MH周围放置前外侧（ALC）或后外侧（PLC）切口后7-8天，在大鼠的选定脑区域中定量免疫反应性ACTH和β-内啡肽（ir-ACTH和ir-beta E）。在ALC后的MH中观察到两种肽的逆行积累，但在PLC后未观察到。ALC导致所有MR外脑区（MR外下丘脑、隔、丘脑、海马、杏仁核和延髓）ir-ACTH/ir-beta E浓度显著降低。相反，IR-ACM和IR-β E水平下降，仅在丘脑和延髓PLC后。目前的数据表明：（a）在下丘脑弓状区合成的ACTH和β E样物质通过神经元通路轴突运输到下丘脑外脑区，主要从MR前外侧、前背侧或前内侧方向离开（甚至某些后内侧或后外侧投射的纤维也会离开MR的某些前方方向）;（B）后部ACTH/β E能投射似乎不太重要，除了丘脑和延髓，在那里它贡献了大约三分之一的肽含量。我们的生化研究为Khachaturian等人[5]描述的大鼠脑中ACTH/β E能投射的详细免疫组织化学图片提供了定量补充数据。(C)1997年爱思唯尔科学公司
To evaluate the relative weight of the ACTH-ergic and beta-endorphin-ergic pathway(s) leaving the medial hypothalamus (MH) in anterior or posterior directions immunoreactive ACTH and beta-endorphin (ir-ACTH and ir-beta E) were quantified in selected brain regions of the rat 7-8 days after placing anterolateral (ALC) or posterolateral (PLC) cut around the MH. Retrograde accumulation of both peptides was observed in the MH after ALC, but not after PLC. ALC resulted in dramatic decrease in ir-ACTH/ir-beta E concentrations in all extra-MR brain regions tested (extra-MR hypothalamus, septum, thalamus, hippocampus, amygdala, and medulla oblongata). In contrast, ir-ACM and ir-beta E levels decreased only in the thalamus and in the medulla oblongata after PLC. The present data indicate (a) ACTH- and beta E-like substances synthesized in the arcuate region of the hypothalamus are axonally transported to extrahypothalamic brain regions by neuronal pathways leaving the MR primarily anterolateral, anterodorsal, or anteromedial direction (even the fibers of certain posteromedial or posterolateral projections leave the MR some anterior directions); (b) the posterior ACTH/beta E-ergic projections seem to be of minor importance except for the thalamus and the medulla oblongata where it contributes to about one-third of the peptide content. Our biochemical study provide quantitative complementary data to the detailed immunohistochemical picture of the ACTH/beta E-ergic projections in the rat brain described by Khachaturian et al. [5]. (C) 1997 Elsevier Science Inc.