The post-transcriptional regulation of LaSCL6 by miR171 during maintenance of embryogenic potential in Larix kaempferi (Lamb.) Carr.
The post-transcriptional regulation of LaSCL6 by miR171 during maintenance of embryogenic potential in Larix kaempferi (Lamb.) Carr.
复制标题
miR171 在 Larix kaempferi (Lamb.) Carr 胚胎发生潜力维持过程中对 LaSCL6 的转录后调控。
DOI：
10.1007/s11295-013-0668-y
复制
发表时间：
2014-02-01
影响因子：
2.4
通讯作者：
Qi, Li-Wang
中科院分区：
文献类型：
--
作者：
Li, Wan-Feng;Zhang, Shou-Gong;Qi, Li-Wang
体细胞胚胎发生为探索决定细胞命运的机制提供了机会。在先前的研究中，在日本落叶松胚性培养和非胚性培养以及体细胞胚胎成熟过程中，发现成熟miR171的水平存在差异。卡尔。然而，人们对其在这些过程中的目标基因知之甚少。在此，克隆了日本稻草人类稻草人6(SCL6)的全长同源基因LaSCL6。序列分析表明，miR171靶序列存在于LaSCL6转录本中。对miRNA引导的LaSCL6切割产物的分离进一步表明，该基因受到miR171的调控。对胚性培养、非胚性培养和体细胞胚胎成熟后期的LaSCL6转录水平进行了测定，结果表明，在胚性培养中，LaSCL6的mRNAs发生了活跃的切割。根据LaSCL6与成熟miR171的表达模式之间的关系，我们认为miR171对LaSCL6的转录后调控可能参与了体细胞胚胎发生潜能的维持，为体细胞胚胎发生的调控机制提供了新的见解。
Somatic embryogenesis provides an opportunity to explore the mechanisms underlying the determination of cell fate. In a previous study, differential levels of mature miR171 were found between embryogenic and non-embryogenic cultures and during somatic embryo maturation in Larix kaempferi (Lamb.) Carr. However, little is known about its target genes in these processes. Here, a full-length cDNA for the SCARECROW-LIKE 6 (SCL6) homolog from L. kaempferi, LaSCL6, was cloned. Sequence analysis showed that the miR171 target sequence was present in the LaSCL6 transcript. Isolation of the miRNA-guided cleavage products of LaSCL6 further suggested that this gene was being regulated by miR171. LaSCL6 transcript levels in embryogenic and non-embryogenic cultures and during the late stage of somatic embryo maturation were measured, and the results showed that cleavage of the LaSCL6 mRNAs occurred actively in embryogenic cultures. Based on the relationships between the expression patterns of LaSCL6 and mature miR171, we concluded that the post-transcriptional regulation of LaSCL6 by miR171 might participate in the maintenance of embryogenic potential, providing new insights into the regulatory mechanisms of somatic embryogenesis.