ENDOCRINE CONTROL OF VITELLOGENESIS IN DROSOPHILA-MELANOGASTER - EFFECTS OF BRAIN AND CORPUS-ALLATUM
ENDOCRINE CONTROL OF VITELLOGENESIS IN DROSOPHILA-MELANOGASTER - EFFECTS OF BRAIN AND CORPUS-ALLATUM
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DOI：
10.1002/jez.1402020309
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发表时间：
1977-01-01
影响因子：
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通讯作者：
POSTLETHWAIT, JH
中科院分区：
文献类型：
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作者：
HANDLER, AM;POSTLETHWAIT, JH
采用结扎法和移植法研究了黑腹金丝猴卵黄沉积的内分泌调控。与卵黄发生相关的内分泌事件与羽化同步，而不是与成虫发育完成同步。断头实验表明，大约在羽化期间发生的头部事件是每种动物启动卵黄发生所必需的。头部的形态发生效应可以被一种保幼激素类似物(JHA)取代。除了头部事件，每个卵泡都需要一个胸部因素来启动卵黄发生，因为在羽化后16小时前准备孤立的腹部可以防止卵黄发生。在此时间之后分离的腹部，没有形成早期的卵黄形成阶段。通过植入翼体或用JHA处理这些制剂，可以逆转对孤立腹部卵黄发生的抑制，但不能通过植入心脏体来逆转。经JHA处理后的人工成熟卵巢经聚丙烯酰胺凝胶电泳法后仍显示正常的卵巢蛋白补体。生物钟在羽化时通过头部信号触发卵黄生成，进而触发胸部或腹部的事件。头部信号可以被JH取代，JH的存在是每个卵泡形成卵黄所必需的。
The endocrine control of yolk deposition in D. melanogaster was studied by ligation and transplantation techniques. Endocrine events associated with the initiation of vitellogenesis were synchronized with eclosion rather than the completion of adult development. Decapitation experiments showed that a cephalic event occurring at about the time of eclosion was necessary for each animal to initiate vitellogenesis. The morphogenetic effect of the head could be replaced by a juvenile hormone analog (JHA). In addition to the cephalic event, a thoracic factor was required for each follicle to initiate vitellogenesis, since preparation of isolated abdomens before 16 h after eclosion prevented vitellogenesis. In abdomens isolated after this time, no early vitellogenic stages were formed. The suppression of vitellogenesis in isolated abdomens was reversed by implanting corpora allata or by treating these preparations with JHA, but not by implanting corpora cardiaca. Ovaries artificially induced to mature by treating isolated abdomens with JHA still displayed the normal complement of ovarian proteins after electrophoresis in polyacrylamide gels. A circadian clock triggered vitellogenesis via a cephalic signal at eclosion, which in turn triggered events in the thorax or abdomen. The cephalic signal could be superseded by JH whose presence was necessary for each follicle to become vitellogenic.