Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme
Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme
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DOI：
10.1021/tx300174e
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发表时间：
2012-06-01
影响因子：
4.1
通讯作者：
Wladyka, Benedykt
中科院分区：
文献类型：
--
作者：
Dziga, Dariusz;Wasylewski, Marcin;Wladyka, Benedykt
将鞘氨醇单胞菌ACM-3962菌株的MlrC蛋白在大肠杆菌BL 21（DE 3）中异源表达并纯化，以研究该酶参与微囊藻毒素的生物降解。与以前的报道相比，我们的研究结果表明，MlrC切割线性微囊藻毒素，从而脱落的MlrC酶在微囊藻毒素生物降解的作用新的光。
The MlrC protein from Sphingomonas ACM-3962 strain was heterologously expressed in Escherichia coli strain BL21(DE3) and purified to investigate participation of this enzyme in the biodegradation of two microcystin variants. In contrast with previous reports, our results indicated that MlrC cleaves linear microcystins, thus shedding new light on the role of MlrC enzyme in microcystin biodegradation.