Quantitation of virus-specific classes of antibodies following immunization of mice with attenuated equine herpesvirus 1 and viral glycoprotein D.
Quantitation of virus-specific classes of antibodies following immunization of mice with attenuated equine herpesvirus 1 and viral glycoprotein D.
复制标题
用减毒马疱疹病毒 1 和病毒糖蛋白 D 免疫小鼠后，对病毒特异性抗体类别进行定量。
DOI：
10.1006/viro.2000.0197
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
O'Callaghan,DJ
中科院分区：
文献类型：
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作者：
Zhang,Y;Smith,PM;Jennings,SR;O'Callaghan,DJ
使用 ELISPOT 测定法测量呼吸道区域淋巴组织中的 EHV-1 特异性抗体分泌细胞 (ASC)，研究鼻内 (i.n.) 感染马疱疹病毒 1 (EHV-1) 减毒 KyA 株或致病性 RacL11 株或重组糖蛋白 D (rgD) 免疫的 CBA/J 小鼠的抗体反应。注射后 2 周，在纵隔淋巴结 (MLN) 和肺部检测到 EHV-1 特异性的 IgG、IgA 和 IgM ASC。 EHV-1 株 KyA 或 RacL11 感染，或用热灭活的 KyA 或 rgD 进行免疫。第 4 周和第 8 周时，EHV-1 特异性 ASC 出现在 MLN 和肺部，但第 8 周时肺部中的频率下降了五倍。然而，i.n. 8周时感染致病性EHV-1 RacL11的免疫小鼠（2×106pfu KyA或50 μg rgD/小鼠）抵抗攻击，并在攻击后3天显示MLN ASC和肺ASC分别增加八倍和十倍。与鼻内免疫途径相反，腹膜内免疫在 MLN 和肺部产生的 ASC 频率仅略高于未免疫对照小鼠的频率。这些数据表明，用感染性或热灭活的 EHV-1 KyA 或 rgD 进行免疫，可在 MLN 和肺部诱导显着水平的病毒特异性 ASC、特定的记忆 B 细胞反应和长期保护性免疫。 EHV-1 致病株与减毒株诱导的 ASC 数量几乎相同，这一发现表明产生 B 细胞反应的能力独立于 EHV-1 毒力，并且与 EHV-1 毒力无关。
The antibody responses of CBA/J mice infected intranasally (i.n.) with either the attenuated KyA strain or the pathogenic RacL11 strain of equine herpesvirus 1 (EHV-1) or immunized with recombinant glycoprotein D (rgD) were investigated using the ELISPOT assay to measure EHV-1-specific antibody-secreting cells (ASC) in the regional lymphoid tissue of the respiratory tract. IgG, IgA, and IgM ASC specific for EHV-1 were detected in the mediastinal lymph nodes (MLN) and lungs 2 weeks after i.n. infection with EHV-1 strain KyA or RacL11, or immunization with heat-killed KyA or rgD. EHV-1-specific ASC were present in the MLN and lungs at 4 and 8 weeks, but declined in frequency by fivefold in the lung at 8 weeks. However, i.n. immunized (2 × 106pfu KyA or 50 μg rgD/mouse) mice infected at 8 weeks with pathogenic EHV-1 RacL11 resisted challenge and showed eight- and tenfold increases in MLN ASC and lung ASC, respectively, by 3 days after challenge. In contrast to the intranasal route of immunization, intraperitoneal immunization yielded ASC frequencies in the MLN and lungs that were only slightly above those of nonimmunized control mice. These data indicate that immunization with infectious or heat-killed EHV-1 KyA, or rgD, induces significant levels of virus-specific ASC both in the MLN and lungs, a specific memory B-cell response, and long-term protective immunity. The finding that the numbers of ASC induced by the pathogenic strain versus the attenuated strain of EHV-1, which were virtually identical, indicated that the ability to generate a B-cell response is independent of and does not contribute to EHV-1 virulence.