Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
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DOI：
10.1111/j.1574-6968.2006.00307.x
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发表时间：
2006-07-01
影响因子：
2.1
通讯作者：
Arraiano, Cecilia M.
中科院分区：
文献类型：
--
作者：
Freire, Patrick;Vieira, Helena L. A.;Arraiano, Cecilia M.
大肠杆菌bolA是一种参与应激反应和细胞分裂的形态基因。bolA过表达诱导生物膜形成，影响羧基肽酶PBP5、PBP6和β -内酰胺酶AmpC的水平。在这项研究中，我们已经证明了bolA表达的变化会导致外膜性质的改变。当bolA过表达时，对洗涤剂和万古霉素的敏感性降低，荧光探针显示不同水平的bolA对外膜蛋白的可及性有影响。此外，研究表明，bolA参与了OmpF/OmpC平衡的调节。
Escherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and beta-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.