Effects of sleep restriction on the human plasma metabolome
Effects of sleep restriction on the human plasma metabolome
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DOI：
10.1016/j.physbeh.2013.08.007
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发表时间：
2013-10-02
影响因子：
2.9
通讯作者：
Penev, Plamen D.
中科院分区：
文献类型：
--
作者：
Bell, Lauren N.;Kilkus, Jennifer M.;Penev, Plamen D.
本研究探讨了反复睡眠限制对有 2 型糖尿病家族风险的成年人血浆代谢组的影响。 11 名父母有 2 型糖尿病史的健康成年人（6 M/5 F；平均 [SD] 年龄：26131 岁；BMI 23.5 [2.3] kg/m(2)）参加了在一家学术医院的临床资源中心进行的一项两条件、两期随机交叉研究。每位参与者都完成了两次为期 8 晚的住院治疗，其中睡眠机会有限（5.5 小时卧床时间）与充分睡眠（8.5 小时卧床时间），同时仔细控制每日食物摄入量和体力活动。使用两个 UHPLC/MS/MS 平台和一个 GC/MS 平台的组合来测量研究参与者在每次 8 晚睡眠治疗后的早晨收集的空腹血浆样本中的 362 种生化物质。当睡眠减少时，12 种氨基酸和相关代谢物的相对浓度会增加。睡眠限制还会导致多种脂肪酸、胆汁酸、类固醇激素和三羧酸循环中间体的升高。相比之下，当睡眠减少时，葡萄糖、一些单糖、葡萄糖酸盐和五碳糖醇的循环水平往往会下降。反复睡眠不足会影响有 2 型糖尿病风险的成年人的多种中间代谢途径。血浆氨基酸和相关生化物质的升高是 8 晚睡眠限制后最明显的代谢特征。 (C) 2013 Elsevier Inc. 保留所有权利。
This study examined the effects of recurrent sleep restriction on the plasma metabolome of adults with familial risk of type 2 diabetes. Eleven healthy adults (6 M/5 F; mean [SD] age: 26131 years; BMI 23.5 [2.3] kg/m(2)) with parental history of type 2 diabetes participated in a two-condition, two-period randomized crossover study at the Clinical Resource Center at an academic hospital. Each participant completed two 8-night inpatient sessions with restricted (5.5-h time-in-bed) vs. adequate (8.5-h time-in-bed) sleep opportunity while daily food intake and physical activity were carefully controlled. A combination of two UHPLC/MS/MS platforms and one GC/MS platform was used to measure 362 biochemicals in fasting plasma samples collected from study participants the morning after each 8-night sleep treatment. Relative concentrations of 12 amino acids and related metabolites were increased when sleep was curtailed. Sleep restriction also induced elevations in several fatty acid, bile acid, steroid hormone, and tricarboxylic acid cycle intermediates. In contrast, circulating levels of glucose, some monosaccharides, gluconate, and five-carbon sugar alcohols tended to decline when sleep was reduced. Recurrent sleep curtailment affected multiple pathways of intermediary metabolism in adults at risk for type 2 diabetes. An elevation in plasma amino acids and related biochemicals was the most pronounced metabolic signature seen in response to 8 nights of sleep restriction. (C) 2013 Elsevier Inc. All rights reserved.