In vitro production of functional sperm in mice.
In vitro production of functional sperm in mice.
复制标题
小鼠功能性精子的体外生产。
DOI：
10.1038/aja.2011.98
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发表时间：
2011
影响因子：
2.9
通讯作者：
Lamb,DoloresJ
中科院分区：
文献类型：
--
作者：
Hwang,Kathleen;Lamb,DoloresJ
精子发生是指精子（大多数有性生殖物种的成熟雄配子）产生和发育的过程。这是一个复杂的，逐步的精子发育过程，需要一个多月的时间，这取决于涉及三个特定功能阶段的物种：精原细胞增殖，减数分裂和变态。研究人员已经成功地在某些鱼类中重现了这一过程;然而，这整个过程从未在哺乳动物的体外重现过。[2，3]在最近一期的《自然》杂志上，Sato和同事们试图用培养的新生小鼠组织在体外产生功能性精子。使用两种不同的转基因小鼠品系，其中包含一个绿色荧光蛋白（GFP）标记，研究人员可以跟踪GFP的相对表达，以确定减数分裂和单倍体细胞的存在。器官培养方法在20世纪60年代取得了惊人的进展，特别是在睾丸培养方面;然而，它不可能促进精子发生超过粗线期阶段。在接下来的几十年里，研究人员专注于细胞培养方法，以实现体外精子发生。尽管做出了这些努力，但进展有限，Sato及其同事重新评估了器官培养方法在体外产生成熟精子的可行性。
Spermatogenesis refers to the process by which the production and development of the spermatozoa (the mature male gamete of most sexually reproducing species) occurs. It is a complex, stepwise process of sperm development, taking more than a month depending upon the species involving three specific functional phases: spermatogonial proliferation, meiosis and metamorphosis. 1 Investigators have been successful in recreating this process in some species of fish; however, this whole process has never been reproduced in vitro in mammals. 2, 3 In a recent issue of Nature, Sato and colleagues4 attempted to produce functional sperm in vitro with cultured neonatal mouse tissue. Using two different transgenic mouse lines that incorporated a green fluorescent protein (GFP) marker, the researchers could follow the relative expression of GFP to define the presence of meiosis and haploid cells. Organ culture methods made spectacular progress in the 1960s, particularly in testicular culture; however, it was not possible to promote spermatogenesis beyond the pachytene stage. 5 Over the next few decades, researchers then focused on cell culture methods to achieve spermatogenesis in vitro. Despite such endeavors, progress was limited and Sato and colleagues re-evaluated the feasibility of organ culture methods to produce mature sperm in vitro.
器官培养中大鼠生精上皮的分化。
DOI：
--
发表时间：
1965
期刊：
Journal of Reproduction and Fertility
影响因子：
--
作者：
A. Steinberger;E. Steinberger
通讯作者：
E. Steinberger
影响因子：
2.8
作者：
J. Parks;D. R. Lee;S. Huang;M. Kaproth
通讯作者：
J. Parks;D. R. Lee;S. Huang;M. Kaproth