Upgrading Fungal Gene Expression on Demand: Improved Systems for Doxycycline-Dependent Silencing in Aspergillus fumigatus
Upgrading Fungal Gene Expression on Demand: Improved Systems for Doxycycline-Dependent Silencing in Aspergillus fumigatus
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DOI：
10.1128/aem.03626-12
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发表时间：
2013-03-01
影响因子：
4.4
通讯作者：
Wagener, Johannes
中科院分区：
文献类型：
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作者：
Helmschrott, Christoph;Sasse, Anna;Wagener, Johannes
条件基因表达是任何特定微生物功能研究的关键。为了在致病霉菌烟曲霉中进行严格调控，通过替换前体模块的功能元件，产生了多西环素依赖性Tet-On系统的改进版本，从而避免了以前由于分子内重组而导致的泄漏问题。
Conditional gene expression is key for functional studies in any given microorganism. To allow tight regulation in the pathogenic mold Aspergillus fumigatus, improved versions of the doxycycline-dependent Tet-On system were generated by replacing functional elements of the precursor module, thereby circumventing the former problem of leakiness due to intramolecular recombination.