Steady-state level of the specific glucocorticoid binding component in mouse fibroblasts.
Steady-state level of the specific glucocorticoid binding component in mouse fibroblasts.
复制标题
小鼠成纤维细胞中特定糖皮质激素结合成分的稳态水平。
DOI：
10.1021/bi00771a010
复制
发表时间：
1972
期刊：
影响因子：
2.9
通讯作者：
Lewis Aronow
中科院分区：
文献类型：
--
作者：
Douglas N. Ishii;William B. Pratt;Lewis Aronow
石井，PRATT和ARONOW摘要：当L细胞在37 ℃下与放射性曲安奈德孵育时，以特定方式结合的类固醇的量在几个小时内保持恒定。Chase实验表明，完整细胞的可溶性部分中的特异性结合组分（受体）持续失活或再循环（半衰期30-40分钟）。新的、未被占据的结合组分通过温度依赖性、能量依赖性的过程不断进入系统，并且不需要同时进行蛋白质合成。7000 g上清液中类固醇和结合组分的复合物与37 ℃下结合到7000 g颗粒级分的类固醇处于平衡。能量剥夺减少了上清液中结合的类固醇，但增加了颗粒部分中的结合类固醇。这种作用是可逆的。体外生长的虱子成纤维细胞（L929）含有糖皮质激素类固醇的结合组分，在低渗破裂后，糖皮质激素类固醇在很大程度上被重新覆盖在细胞的可溶性部分中（哈克尼等人，1970年）。通过Sephadex G-200过滤测定，该分子或分子聚集体的表观分子量为620，000，并已纯化约2000倍（哈克尼和普拉特，1971）。
ISHII, PRATT, AND ARONOW abstract: When L cells are incubated at 37 in the presence of radioactive triamcinolone acetonide, the amount of steroid bound in a specific manner remains constant for several hours. Chase experiments reveal that the specific binding component (receptor) in the soluble fraction of the intact cell is being continually inactivated or recycled (half-time 30-40 min). New, unoccupied binding component is continually entering the system by a process that is temperature dependent, energy dependent, and does not require simultaneous protein synthesis. The complex of steroid and binding component in the 7000g supernatant is in equilibrium with steroid bound to the 7000g particulatefraction at 37. Energy deprivation reduces the bound steroidin the supernatant fraction but increases it in the particulate fraction. This effect is reversibleJ^^ louse fibroblasts (L929) growing in vitro contain a binding component for glucocorticoid steroids which is re-covered largely in the soluble fraction of the cell after hypotonic rupture (Hackney et al., 1970). This molecule or molecu-lar aggregate has an apparent molecular weight of 620,000 as determined by filtration through Sephadex G-200, and has been purified approximately 2000-fold (Hackney and Pratt, 1971).