Accelerating Bone Healing by Decorating BMP-2 on Porous Composite Scaffolds
Accelerating Bone Healing by Decorating BMP-2 on Porous Composite Scaffolds
复制标题
通过在多孔复合支架上装饰 BMP-2 加速骨愈合
DOI：
10.1021/acsabm.9b00761
复制
发表时间：
2019-12-16
影响因子：
4.7
通讯作者：
Li, Ji-Hua
中科院分区：
文献类型：
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作者：
Li, Xiang;Yin, Hua-Mo;Li, Ji-Hua
尽管人工聚合物支架在骨修复中起着至关重要的作用，但其应用因生物活性较差而受到限制。在此，通过同时引入具有生物活性的纳米羟基磷灰石（nHA）、生物玻璃（BG）以及与聚多巴胺（pDA）结合的骨形态发生蛋白 - 2（BMP - 2），制备了具有骨诱导性的聚（ε - 己内酯）（PCL）复合支架。研究发现，pDA沉积不仅显著提高了复合支架的亲水性和细胞亲和力，还使BMP - 2能够稳定固定并实现长期持续释放。与纯PCL和PCL/nHA/BG（PHB）支架相比，所设计的PHB - pDA - BMP - 2支架显著诱导骨髓基质细胞向成骨细胞系分化。同时，体内实验表明PHB - pDA - BMP - 2支架具有显著的成骨能力，能够使兔子颅骨缺损在12周内完全愈合。这一发现表明，所开发的多孔复合支架在骨再生方面具有巨大潜力。
Although artificial polymeric scaffolds act as vital characters in bone repair, their application is limited due to their inferior bioactivity. Herein, osteoinductive poly(e-caprolactone) (PCL) composite scaffolds were prepared by synchronously enlisting bioactive nanohydroxyapatite (nHA), bioglass (BG), and bone morphogenetic protein-2 (BMP-2), which was bound up with polydopamine (pDA). It was found that pDA deposition not only significantly enhanced hydrophilicity and cell affinity of composite scaffolds but also endowed steady immobilization of BMP-2 with long-term yet sustained release. Compared to pure PCL and PCL/nHA/BG (PHB) scaffolds, the designed PHB-pDA-BMP-2 scaffolds significantly induced the differentiation of bone marrow stromal cells toward an osteogenic lineage. Meanwhile, in vivo examinations revealed the prominent osteogenic capability of PHB-pDA-BMP-2 scaffolds, which enabled complete bone healing of calvarial bone defects in rabbits by 12 weeks. This finding indicates that the developed porous composite scaffolds hold great potential for bone regeneration.