In vitro metastatic colonization of human ovarian cancer cells to the omentum
In vitro metastatic colonization of human ovarian cancer cells to the omentum
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DOI：
10.1007/s10585-010-9317-0
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发表时间：
2010-03-01
影响因子：
4
通讯作者：
Rinker-Schaeffer, Carrie W.
中科院分区：
文献类型：
--
作者：
Khan, Shaheena M.;Funk, Holly M.;Rinker-Schaeffer, Carrie W.
尽管网膜在调节卵巢癌转移性生长中可能具有重要作用，但它仍然是一个知之甚少的器官。由于其解剖位置和结构脆弱性，网膜对基于机制的体内研究提出了固有的挑战。因此，离体网膜模型的可用性将部分解决这些困难。在这里，我们描述了一种技术，用于识别，分离和维持离体培养的网膜免疫功能低下和有能力的小鼠。开发了维持组织活力、结构和功能长达10天的离体培养条件。进一步的实验表明，离体培养条件允许卵巢癌细胞在体外增殖，并支持腹膜内注射卵巢癌细胞或卵巢癌细胞与网膜共培养后的相似模式的显微病变。与我们实验室以前的研究一致，这些标本的组织学评价发现，卵巢癌细胞以及其他腹膜癌细胞优先在富含免疫细胞的网膜区域积聚并定植。我们现在认识到，这些是特定的功能性结构，称为乳斑。总之，这些都是一个容易获得的模型，这是很容易操作，可以立即用于研究网膜殖民的动态过程的基础研究。希望研究人员将使用本文中的数据作为改进和修改的起点，这将使他们能够根据他们希望追求的实验问题的特定需求定制模型。
Despite the potentially crucial contributions of the omentum in the regulation of ovarian cancer metastatic growth, it remains a poorly understood organ. Due to its anatomic location and structural fragility, the omentum presents inherent challenges to mechanism-based in vivo studies. Thus, the availability of an ex vivo omental model would, in part, address some of these difficulties posed. Here we describe a technique for identifying, isolating and maintaining ex vivo cultures of omenta from immune-compromised and -competent mice. Ex vivo culture conditions were developed that maintain tissue viability, architecture, and function for up to 10 days. Further experiments demonstrate that the ex vivo culture conditions allow for the proliferation of ovarian cancer cells in vitro and support a similar pattern of microscopic lesions after either intraperitoneal injection of ovarian cancer cells or co-culture of ovarian cancer cells with the omentum. In agreement with previous studies from our laboratory, histologic evaluation of these specimens found that ovarian cancer cells, as well as other peritoneal cancer cells, preferentially accumulate in, and colonize, omental areas rich in immune cells. We now recognize that these are specific, functional structures referred to as milky spots. In sum, these are foundational studies of a readily accessible model, which is easily manipulated and can be immediately used to study the dynamic process of omental colonization. It is hoped that investigators will use the data herein as a starting point for refinements and modifications which will enable them to tailor the model to the specific needs of the experimental question(s) they wish to pursue.