Capillary electrophoresis chips with integrated electrochemical detection
Capillary electrophoresis chips with integrated electrochemical detection
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DOI：
10.1021/ac971135z
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发表时间：
1998-02-15
影响因子：
7.4
通讯作者：
Mathies, RA
中科院分区：
文献类型：
--
作者：
Woolley, AT;Lao, KQ;Mathies, RA
集成电化学检测的毛细管电泳系统已经在玻璃基板上微制造出来。光刻法将工作电极放置在电泳通道出口外，提供了高灵敏度的电化学检测，分离电场的干扰最小。微芯片电泳分离神经递质在100以下的例子说明了这些设备的良好的分辨率和原子检测灵敏度。采用间接电化学检测，还进行了高灵敏度DNA限制性片段和PCR产物的分级。这些微型装置将探测器的尺寸与微制造分离和反应装置的尺寸相匹配，使芯片实验室的概念成为现实。
Capillary electrophoresis systems with integrated electrochemical detection have been microfabricated on glass substrates. Photolithographic placement of the working electrode just outside the exit of the electrophoresis channel provides high-sensitivity electrochemical detection with minimal interference from the separation electric field. Microchip electrophoretic separations of neurotransmitters in under 100 exemplify the good resolution and attomole detection sensitivity of these devices. Using indirect electrochemical detection, high-sensitivity DNA restriction fragment and PCR product sizing has also been performed. These microdevices match the detector's size to that of microfabricated separation and reaction devices, bringing to reality the lab-on-a-chip concept.