CcdA Is a Thylakoid Membrane Protein Required for the Transfer of Reducing Equivalents from Stroma to Thylakoid Lumen in the Higher Plant Chloroplast
CcdA Is a Thylakoid Membrane Protein Required for the Transfer of Reducing Equivalents from Stroma to Thylakoid Lumen in the Higher Plant Chloroplast
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DOI：
10.1089/ars.2010.3138
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发表时间：
2010-10-01
影响因子：
6.6
通讯作者：
Hisabori, Toru
中科院分区：
文献类型：
--
作者：
Motohashi, Ken;Hisabori, Toru
为了将还原当量转移到类囊体腔中，建议一种特定的类囊体膜转移系统介导高等植物叶绿体类囊体腔中蛋白质的二硫键还原。在这个系统中，虽然基质硫氧还蛋白可以提供还原当量的类囊体腔中的硫氧还蛋白样蛋白HCF 164，介体蛋白类囊体膜中的电子转移被建议需要连接两个亚细胞器室。CcdA是一个候选蛋白作为该转移系统的一个组成部分，因为CcdA-和HCF 164-缺陷突变体在拟南芥中表现出相同的表型。我们现在表明，CcdA是本地化的类囊体膜和它的氧化还原状态，以及HCF 164，调制在类囊体基质M-型硫氧还蛋白。我们的研究结果强烈表明，CcdA可能作为一个调解人在类囊体膜的减少等价物从基质转移到叶绿体类囊体膜的内腔侧。抗氧化剂。氧化还原信号。13，1169-1176.
In order to transfer reducing equivalents into the thylakoid lumen, a specific thylakoid membrane transfer system is suggested that mediates the disulfide bond reduction of proteins in the thylakoid lumen of higher plant chloroplasts. In this system, although stromal thioredoxin can supply the reducing equivalents to a thioredoxin-like protein HCF164 in the thylakoid lumen, a mediator protein for electron transfer in the thylakoid membranes is proposed to be required to link the two suborganellar compartments. CcdA is a candidate protein as a component for this transfer system since CcdA- and HCF164-deficient mutants in Arabidopsis thaliana show the same phenotype. We now show that CcdA is localized in the thylakoid membrane and that its redox state, as well as that of HCF164, is modulated in thylakoids by stromal m-type thioredoxin. Our results strongly suggest that CcdA may act as a mediator in thylakoid membranes by transferring reducing equivalents from the stromal to the lumenal side of the thylakoid membrane in chloroplasts. Antioxid. Redox Signal. 13, 1169-1176.