Evolution of early embryogenesis in rhabditid nematodes
Evolution of early embryogenesis in rhabditid nematodes
复制标题
DOI：
10.1016/j.ydbio.2009.07.033
复制
发表时间：
2009-11-01
影响因子：
2.7
通讯作者：
Piano, Fabio
中科院分区：
文献类型：
--
作者：
Brauchle, Michael;Kiontke, Karin;Piano, Fabio
指导早期胚胎发育的细胞生物学事件在物种内非常精确地发生，但在物种之间可能非常不同。这些细胞过程是如何进化的，以及哪些分子成分是进化变化的基础，目前还知之甚少。为了开始解决这些问题，我们系统地研究了早期胚胎发生，从一个到四个细胞胚胎，在34个线虫物种有关的C。优雅的。我们发现了40个细胞生物学特征，捕捉这些物种之间的表型差异。通过追踪分子遗传学上的进化变化，我们发现这些特征经历了多次进化，并且彼此独立。引人注目的是，所有这些表型都可以通过秀丽隐杆线虫单基因RNA干扰实验来模拟。我们使用这些比较来假设进化变化背后的分子机制。例如，我们预测，细胞极性模块被改变在原小杆线虫组的进化过程中，并显示PAR-1，激酶定位不对称的线虫早期胚胎，是对称地定位在原小杆线虫组物种的单细胞阶段。我们的全基因组方法确定了与细胞生物学表型进化变化相关的候选分子，从而确定了模块。(C)2009 Elsevier Inc. All rights reserved.
The cell-biological events that guide early-embryonic development occur with great precision within species but can be quite diverse across species. How these cellular processes evolve and which molecular components underlie evolutionary changes is poorly understood. To begin to address these questions, we systematically investigated early embryogenesis, from the one- to the four-cell embryo, in 34 nematode species related to C. elegans. We found 40 cell-biological characters that captured the phenotypic differences between these species. By tracing the evolutionary changes on a molecular phylogeny, we found that these characters evolved multiple times and independently of one another. Strikingly, all these phenotypes are mimicked by single-gene RNAi experiments in C elegans. We use these comparisons to hypothesize the molecular mechanisms underlying the evolutionary changes. For example, we predict that a cell polarity module was altered during the evolution of the Protorhabditis group and show that PAR-1, a kinase localized asymmetrically in C elegans early embryos, is symmetrically localized in the one-cell stage of Protorhabditis group species. Our genome-wide approach identifies candidate molecules-and thereby modules-associated with evolutionary changes in cell-biological phenotypes. (C) 2009 Elsevier Inc. All rights reserved.