The effect of ultraviolet C radiation against different N95 respirators inoculated with SARS-CoV-2
The effect of ultraviolet C radiation against different N95 respirators inoculated with SARS-CoV-2
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DOI：
10.1016/j.ijid.2020.08.077
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发表时间：
2020-11-01
影响因子：
8.4
通讯作者：
Mi, Qing-Sheng
中科院分区：
文献类型：
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作者：
Ozog, David M.;Sexton, Jonathan Z.;Mi, Qing-Sheng
目的：目前还没有研究表明，对于不同类型的口罩，同一剂量是否可以有效地去污N95滤式口罩(FFR)上的SARS-CoV-2。在大流行期间，卫生保健工作者一直在使用这种消毒方法。方法：在5种不同的N95 FFR模型(3M1860、8210、8511、9211；Moldex 1511)上的4个不同部位(面罩和背带)接种10亩L滴剂(8×10(7)TCID50/mL)。口罩外表面和佩戴者面对表面分别照射1.5J/cm(2)UVC(254 Nm)剂量。结果：对3M 1860和Moldex 1511的面罩，以及3M 8210和Moldex 1511的条带，使用1.5J/cm(2)的紫外线照射是一种有效的去污方法。结论：该剂量是一种合适的去污方法，便于医护人员在使用特定型号/材料时重复使用呼吸器。此外，有些带子可能需要额外消毒，以最大限度地保障前线工作人员的安全。广泛使用的紫外线去污方法的实施需要仔细考虑模型、材料类型、设计和辐照后的适合性测试。(C)爱思唯尔有限公司代表国际传染病学会出版的《2020》。
Objectives: There are currently no studies that have examined whether one dosage can be uniformly applied to different respirator types to effectively decontaminate SARS-CoV-2 on N95 filtering facepiece respirators (FFRs). Health care workers have been using this disinfection method during the pandemic. Our objective was to determine the effect of UVC on SARS-CoV-2 inoculated N95 respirators and whether this was respirator material/model type dependent.Methods: Four different locations (facepiece and strap) on five different N95 FFR models (3M 1860, 8210, 8511, 9211; Moldex 1511) were inoculated with a 10 mu L drop of SARS-CoV-2 viral stock (8 x 10(7) TCID50/mL). The outside-facing and wearer-facing surfaces of the respirators were each irradiated with a dose of 1.5 J/cm(2) UVC (254 nm). Viable SARS-CoV-2 was quantified by a median tissue culture infectious dose assay (TCID50).Results: UVC delivered using a dose of 1.5 J/cm(2), to each side, was an effective method of decontamination for the facepieces of 3M 1860 and Moldex 1511, and for the straps of 3M 8210 and the Moldex 1511.Conclusion: This dose is an appropriate decontamination method to facilitate the reuse of respirators for healthcare personnel when applied to specific models/materials. Also, some straps may require additional disinfection to maximize the safety of frontline workers. Implementation of widespread UVC decontamination methods requires careful consideration of model, material type, design, and fit-testing following irradiation. (C) 2020 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.