WAVELENGTH DEPENDENT FORMATION OF THYMINE DIMERS AND (6-4)PHOTOPRODUCTS IN DNA BY MONOCHROMATIC ULTRAVIOLET-LIGHT RANGING FROM 150 TO 365 NM
WAVELENGTH DEPENDENT FORMATION OF THYMINE DIMERS AND (6-4)PHOTOPRODUCTS IN DNA BY MONOCHROMATIC ULTRAVIOLET-LIGHT RANGING FROM 150 TO 365 NM
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DOI：
10.1111/j.1751-1097.1991.tb02034.x
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发表时间：
1991-09-01
影响因子：
3.3
通讯作者：
NIKAIDO, O
中科院分区：
文献类型：
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作者：
MATSUNAGA, T;HIEDA, K;NIKAIDO, O
我们研究了波长依赖性的环丁烷胸腺嘧啶二聚体和（6-4）的光产物诱导的单色紫外线在该地区从150至365 nm的延伸，使用酶联免疫吸附测定与两个单克隆抗体。小牛胸腺DNA溶液用来自光谱仪的254-365 nm单色UV或来自同步辐射的220-300 nm单色UV照射。胸腺嘧啶二聚体和（6-4）光产物的荧光强度依赖性诱导的每个UV波长到目前为止测试。我们还研究了在干燥条件下由低于220 nm延伸至150 nm的UV对两种类型的DNA损伤的诱导。我们检测到在较短的UV区域以及260 nm处有效形成两种类型的损伤，260 nm被认为是形成UV损伤的最有效波长。胸腺嘧啶二聚体和（6-4）光产物诱导的作用光谱在180 - 300 nm之间相似，而胸腺嘧啶二聚体诱导的作用光谱值在160 nm以下和313 nm以上分别比（6-4）光产物诱导的作用光谱值高约9倍和1.4倍或更多。
We investigated the wavelength dependence of cyclobutane thymine dimer and (6-4) photoproduct induction by monochromatic UV in the region extending from 150 to 365 nm, using an enzyme-linked immunosorbent assay with two monoclonal antibodies. Calf thymus DNA solution was irradiated with 254-365 nm monochromatic UV from a spectrograph, or with 220-300 nm monochromatic UV from synchrotron radiation. Thymine dimers and (6-4)photoproducts were fluence-dependently induced by every UV wavelength tested so far. We also examined the induction of both types of DNA damage by UV below 220 nm extending to 150 nm under dry condition. We detected the efficient formation of both types of damage in the shorter UV region, as well as at 260 nm, which had been believed to be the most efficient wavelength for the formation of UV lesions. The action spectra for the induction of thymine dimers and (6-4)photoproducts were similar from 180 to 300 nm, whereas the action spectrum values for thymine dimer induction were about 9- and 1.4-fold or more higher than the values for (6-4)photoproduct induction below 160 nm and above 313 nm, respectively.